Publication: Cryptic antifungal compounds active by synergism with polyene antibiotics
Issued Date
2016-04-01
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ISSN
13474421
13891723
13891723
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2-s2.0-84940093145
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Bioscience and Bioengineering. Vol.121, No.4 (2016), 394-398
Suggested Citation
Hiroshi Kinoshita, Mariko Yoshioka, Fumio Ihara, Takuya Nihira Cryptic antifungal compounds active by synergism with polyene antibiotics. Journal of Bioscience and Bioengineering. Vol.121, No.4 (2016), 394-398. doi:10.1016/j.jbiosc.2015.08.003 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/43056
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Cryptic antifungal compounds active by synergism with polyene antibiotics
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Abstract
© 2015 The Society for Biotechnology, Japan. The majority of antifungal compounds reported so far target the cell wall or cell membrane of fungi, suggesting that other types of antibiotics cannot exert their activity because they cannot penetrate into the cells. Therefore, if the permeability of the cell membrane could be enhanced, many antibiotics might be found to have antifungal activity. We here used the polyene antibiotic nystatin, which binds to ergosterol and forms pores at the cell membrane, to enhance the cellular permeability. In the presence of nystatin, many culture extracts from entomopathogenic fungi displayed antifungal activity. Among all the active extracts, two active components were purified and identified as helvolic acid and terramide A. Because the minimum inhibitory concentration of either compound was reduced four-fold in the presence of nystatin, it can be concluded that this screening method is useful for detecting novel antifungal activity.