Publication: Intestinal mucosal changes and upregulated calcium transporter and FGF-23 expression during lactation: Contribution of lactogenic hormone prolactin
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Issued Date
2016-01-15
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10960384
00039861
00039861
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2-s2.0-84949486016
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Mahidol University
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SCOPUS
Bibliographic Citation
Archives of Biochemistry and Biophysics. Vol.590, (2016), 109-117
Suggested Citation
Kannikar Wongdee, Jarinthorn Teerapornpuntakit, Chanakarn Sripong, Asma Longkunan, Wasutorn Chankamngoen, Chutiya Keadsai, Kamonshanok Kraidith, Nateetip Krishnamra, Narattaphol Charoenphandhu Intestinal mucosal changes and upregulated calcium transporter and FGF-23 expression during lactation: Contribution of lactogenic hormone prolactin. Archives of Biochemistry and Biophysics. Vol.590, (2016), 109-117. doi:10.1016/j.abb.2015.11.038 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/43149
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Title
Intestinal mucosal changes and upregulated calcium transporter and FGF-23 expression during lactation: Contribution of lactogenic hormone prolactin
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Abstract
© 2015 Elsevier Inc. All rights reserved. As the principal lactogenic hormone, prolactin (PRL) not only induces lactogenesis but also enhances intestinal calcium absorption to supply calcium for milk production. How the intestinal epithelium res-ponses to PRL is poorly understood, but it is hypothesized to increase mucosal absorptive surface area and calcium transporter expression. Herein, lactating rats were found to have greater duodenal, jejunal and ileal villous heights as well as cecal crypt depths than age-matched nulliparous rats. Morphometric analyses in the duodenum and cecum showed that their mucosal adaptations were diminished by bromocriptine, an inhibitor of pituitary PRL release. PRL also upregulated calcium transporter expression (e.g., TRPV6 and PMCA1b) in the duodenum of lactating rats. Since excessive calcium absorption could be detrimental to lactating rats, local negative regulator of calcium absorption, e.g., fibroblast growth factor (FGF)-23, should be increased. Immunohistochemistry confirmed the upregulation of FGF-23 protein expression in the duodenal and cecal mucosae of lactating rats, consistent with the enhanced FGF-23 mRNA expression in Caco-2 cells. Bromocriptine abolished this lactation-induced FGF-23 expression. Additionally, FGF-23 could negate PRL-stimulated calcium transport across Caco-2 monolayer. In conclusion, PRL was responsible for the lactation-induced mucosal adaptations, which were associated with compensatory increase in FGF-23 expression probably to prevent calcium hyperabsorption.