Publication: Novel bioreactor platform for scalable cardiomyogenic differentiation from pluripotent stem cell-derived embryoid bodies
Issued Date
2016-01-01
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ISSN
10643745
Other identifier(s)
2-s2.0-84990230657
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Mahidol University
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SCOPUS
Bibliographic Citation
Methods in Molecular Biology. Vol.1502, (2016), 169-179
Suggested Citation
Sasitorn Rungarunlert, Joao N. Ferreira, Andras Dinnyes Novel bioreactor platform for scalable cardiomyogenic differentiation from pluripotent stem cell-derived embryoid bodies. Methods in Molecular Biology. Vol.1502, (2016), 169-179. doi:10.1007/7651_2016_341 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/43200
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Title
Novel bioreactor platform for scalable cardiomyogenic differentiation from pluripotent stem cell-derived embryoid bodies
Abstract
© Springer Science+Business Media New York 2016. Generation of cardiomyocytes from pluripotent stem cells (PSCs) is a common and valuable approach to produce large amount of cells for various applications, including assays and models for drug development, cell-based therapies, and tissue engineering. All these applications would benefit from a reliable bioreactorbased methodology to consistently generate homogenous PSC-derived embryoid bodies (EBs) at a large scale, which can further undergo cardiomyogenic differentiation. The goal of this chapter is to describe a scalable method to consistently generate large amount of homogeneous and synchronized EBs from PSCs. This method utilizes a slow-turning lateral vessel bioreactor to direct the EB formation and their subsequent cardiomyogenic lineage differentiation.