Publication: Buffered delivery of phosphate to Arabidopsis alters responses to low phosphate
Issued Date
2018-02-23
Resource Type
ISSN
14602431
00220957
00220957
Other identifier(s)
2-s2.0-85042585537
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Experimental Botany. Vol.69, No.5 (2018), 1207-1219
Suggested Citation
Meredith T. Hanlon, Swayamjit Ray, Patompong Saengwilai, Dawn Luthe, Jonathan P. Lynch, Kathleen M. Brown Buffered delivery of phosphate to Arabidopsis alters responses to low phosphate. Journal of Experimental Botany. Vol.69, No.5 (2018), 1207-1219. doi:10.1093/jxb/erx454 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/44814
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Buffered delivery of phosphate to Arabidopsis alters responses to low phosphate
Other Contributor(s)
Abstract
© The Author(s) 2018. Published by Oxford University Press on behalf of the Society for Experimental Biology. Arabidopsis has been reported to respond to phosphate (Pi) stress by arresting primary root growth and increasing lateral root branching. We developed a system to buffer Pi availability to Arabidopsis in gel media systems by charging activated aluminum oxide particles with low and sufficient concentrations of Pi, based on previous work in horticultural and sand culture systems. This system more closely mimics soil chemistry and results in different growth and transcriptional responses to Pi stress compared with plants grown in standard gel media. Low Pi availability in buffered medium results in reduced root branching and preferential investment of resources in axial root growth. Root hair length and density, known responses to Pi stress, increase in low-buffered Pi medium. Plants grown under buffered Pi conditions have different gene expression profiles of canonical Pi stress response genes as compared with their unbuffered counterparts. The system also eliminates known complications with iron (Fe) nutrition. The growth responses of Arabidopsis supplied with buffered Pi indicate that the widely accepted low-Pi phenotype is an artifact of the standard gel-based growth system. Buffering Pi availability through the method presented here will improve the utility and accuracy of gel studies by more closely approximating soil conditions.