Publication: Effective Aeromonas specific monoclonal antibody for immunodiagnosis
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Issued Date
2018-01-01
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ISSN
22211691
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2-s2.0-85041708996
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Mahidol University
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SCOPUS
Bibliographic Citation
Asian Pacific Journal of Tropical Biomedicine. Vol.7, No.1 (2018), 7-13
Suggested Citation
Yuvadee Mahakunkijcharoen, Chakrit Hirunpetcharat, Sunisa Malijunbua, Watcharamat Muangkaew, Suporn Paksanont Effective Aeromonas specific monoclonal antibody for immunodiagnosis. Asian Pacific Journal of Tropical Biomedicine. Vol.7, No.1 (2018), 7-13. doi:10.4103/2221-1691.221128 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/45283
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Title
Effective Aeromonas specific monoclonal antibody for immunodiagnosis
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Abstract
© 2018 Asian Pacific Journal of Tropical Biomedicine Produced by Wolters Kluwer- Medknow. Objective: To identify the monoclonal antibody specific to Aeromonas spp., a Gram negative bacteria causing gastroenteritis and wound infection. Methods: The monoclone, namely 88F2-3F4, was produced from hybridoma technology. The specificity of antibody secreted from 88F2-3F4 was tested against other Gram negative bacteria frequently found in gastrointestinal tract. Then the antibody was used for searching Aeromonas antigens in artificial seeded rectal swab cultures by dot-blot enzyme linked immunosorbent assay. Results: 88F2-3F4 produced an antibody that recognized an antigen with a molecular mass of 8.5 kDa in all 123 isolates of the seven Aeromonas species tested, but recognized no epitope of any other Gram-negative bacterium typically found in the gastrointestinal tract. A dot-blot enzyme linked immunosorbent assay based on this antibody showed 86.49% sensitivity and 92.13% specificity. Conclusions: 88F2-3F4 monoclonal antibody could react with all Aeromonas isolates, but not other Gram negative bacteria, therefore it should be a useful tool for the detection of Aeromonas antigen in clinical and environmental samples.