Publication: Enrichment of human Vγ9Vδ2 T lymphocytes by magnetic poly(divinylbenzene-: Co -glycidyl methacrylate) colloidal particles conjugated with specific antibody
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Issued Date
2018-01-01
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ISSN
20462069
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2-s2.0-85045836662
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Mahidol University
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SCOPUS
Bibliographic Citation
RSC Advances. Vol.8, No.26 (2018), 14393-14400
Suggested Citation
Piamsiri Sawaisorn, Tienrat Tangchaikeeree, Duangporn Polpanich, Panuwat Midoeng, Rachanee Udomsangpetch, Abdelhamid Elaissari, Kulachart Jangpatarapongsa Enrichment of human Vγ9Vδ2 T lymphocytes by magnetic poly(divinylbenzene-: Co -glycidyl methacrylate) colloidal particles conjugated with specific antibody. RSC Advances. Vol.8, No.26 (2018), 14393-14400. doi:10.1039/c8ra01468j Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/45460
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Title
Enrichment of human Vγ9Vδ2 T lymphocytes by magnetic poly(divinylbenzene-: Co -glycidyl methacrylate) colloidal particles conjugated with specific antibody
Abstract
© 2018 The Royal Society of Chemistry. γδ T cells play a significant role in protection against cancer. Purification of γδ T cells is needed for insight when studying their anti-cancer functionality and their utilization in adoptive cell therapy. To improve the purification of γδ T cells, in this work, a composite material based on magnetic nanoparticles was developed for purification of Vγ9Vδ2 T cells, the predominant subset of γδ T lymphocytes in human peripheral blood. The epoxy-functionalized magnetic poly(divinylbenzene-co-glycidyl methacrylate) particles (mPDGs) were bio-conjugated with anti-human Vδ2 antibody to provide specific recognition sites for T cell receptors of Vγ9Vδ2 T cells. Using fluorescence-activated cell sorting (FACS) analysis, separation of Vγ9Vδ2 T cells from peripheral blood mononuclear cells of healthy donors was confirmed with high purity [89.77% (range 87.00-91.80, n = 3)]. More interestingly, the immobilized particles did not affect the viability of purified cells as high cell viability was indicated (>90%). By combining the properties of magnetic nanoparticles with specific antibodies, these immobilized particles were shown to be used as a cell-friendly purification tool of Vγ9Vδ2 T lymphocytes without any limits for the further use of cells. The purified Vγ9Vδ2 T cells using the antibody-immobilized epoxy-functionalized mPDGs could be used directly without a detachment step for further cultivation and expansion. This highlights the advantages of this method in allowing the study of cell function and further investigation of such rare T cell populations in immunotherapy.