Publication: Colorimetric Measurements of Amylase Activity: Improved Accuracy and Efficiency with a Smartphone
5
Issued Date
2018-01-09
Resource Type
ISSN
19381328
00219584
00219584
Other identifier(s)
2-s2.0-85040357847
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Chemical Education. Vol.95, No.1 (2018), 141-145
Suggested Citation
Manchuta Dangkulwanich, Kaness Kongnithigarn, Nattapat Aurnoppakhun Colorimetric Measurements of Amylase Activity: Improved Accuracy and Efficiency with a Smartphone. Journal of Chemical Education. Vol.95, No.1 (2018), 141-145. doi:10.1021/acs.jchemed.7b00468 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/45515
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Colorimetric Measurements of Amylase Activity: Improved Accuracy and Efficiency with a Smartphone
Other Contributor(s)
Abstract
© 2017 The American Chemical Society and Division of Chemical Education, Inc. Routinely used in quantitative determination of various analytes, UV-vis spectroscopy is commonly taught in undergraduate chemistry laboratory courses. Because the technique measures the absorbance of light through the samples, losses from reflection and scattering by large molecules interfere with the measurement. To emphasize the importance of light scattering in UV-vis spectroscopy, a laboratory experiment is described that guides students to study the hydrolysis of starch by amylase as a function of temperature. The reducing sugar produced from the hydrolysis is determined by dinitrosalicylic colorimetric assay via two methods: UV-vis spectroscopy and a smartphone-based colorimetry, which is capable of quantifying the concentration of a chromogenic compound in an opaque sample. From this experiment, students can directly learn to measure the activity of amylase, use a smartphone to perform colorimetry, observe the effect of light scattering, and gain a better understanding of UV-vis spectroscopy.