Publication: Combined Bisulfite Restriction Analysis for brain tissue identification
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Issued Date
2018-05-01
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ISSN
18726283
03790738
03790738
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2-s2.0-85043981703
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Mahidol University
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SCOPUS
Bibliographic Citation
Forensic Science International. Vol.286, (2018), 42-45
Suggested Citation
Jarunya Samsuwan, Tachapol Muangsub, Pattamawadee Yanatatsaneejit, Apiwat Mutirangura, Nakarin Kitkumthorn Combined Bisulfite Restriction Analysis for brain tissue identification. Forensic Science International. Vol.286, (2018), 42-45. doi:10.1016/j.forsciint.2018.02.032 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/46714
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Title
Combined Bisulfite Restriction Analysis for brain tissue identification
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Abstract
© 2018 Elsevier B.V. According to the tissue-specific methylation database (doi: 10.1016/j.gene.2014.09.060), methylation at CpG locus cg03096975 in EML2 has been preliminarily proven to be specific to brain tissue. In this study, we enlarged sample size and developed a technique for identifying brain tissue in aged samples. Combined Bisulfite Restriction Analysis—for EML2 (COBRA-EML2) technique was established and validated in various organ samples obtained from 108 autopsies. In addition, this technique was also tested for its reliability, minimal DNA concentration detected, and use in aged samples and in samples obtained from specific brain compartments and spinal cord. COBRA-EML2 displayed 100% sensitivity and specificity for distinguishing brain tissue from other tissues, showed high reliability, was capable of detecting minimal DNA concentration (0.015 ng/μl), could be used for identifying brain tissue in aged samples. In summary, COBRA-EML2 is a technique to identify brain tissue. This analysis is useful in criminal cases since it can identify the vital organ tissues from small samples acquired from criminal scenes. The results from this analysis can be counted as a medical and forensic marker supporting criminal investigations, and as one of the evidences in court rulings.