Publication: P-gp modulating effect of Azadirachta indica extract in multidrug-resistant cancer cell lines
Issued Date
2018-01-01
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ISSN
00317144
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2-s2.0-85041486353
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Mahidol University
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SCOPUS
Bibliographic Citation
Pharmazie. Vol.73, No.2 (2018), 104-109
Suggested Citation
M. Kawami, Y. Yamada, O. Issarachot, V. B. Junyaprasert, R. Yumoto, M. Takano P-gp modulating effect of Azadirachta indica extract in multidrug-resistant cancer cell lines. Pharmazie. Vol.73, No.2 (2018), 104-109. doi:10.1691/ph.2018.7116 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/47332
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Title
P-gp modulating effect of Azadirachta indica extract in multidrug-resistant cancer cell lines
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Abstract
The extract of Azadirachta indica, commonly known as neem, has found extensive use in traditional medicine for treating various human diseases. In this study, the effect of the 50% ethanol extract of A. indica (AI01) on P-glycoprotein (P-gp)-mediated multidrug resistance (MDR) was examined using MDR cell lines, specifically paclitaxel-resistant HepG2 (PR-HepG2) and doxorubicin (DOX)-resistant (DR) colon-26 cells. 96-h treatment of the two cell lines with AI01 (30 μg/mL) showed no effect on the expression of P-gp mRNA (human MDR1 and mouse mdr1b) and protein, while AI01 increased the accumulation of rhodamine 123, a P-gp substrate, in both PR-HepG2 and DR-colon-26 cells. The cytotoxic effects of 48-h treatment with AI01 on the viability of PR-HepG2 and DR-colon-26 cells were not observed. Therefore, 30 μg/mL AI01 may have no cytotoxic and P-gp-inducing effects. Finally, AI01 potentiated the sensitivity of PR-HepG2 and DR-colon-26 cell lines to DOX by 8.6- and 15.3-fold, respectively. These findings suggest that A. indica may be a promising source for a new class of P-gp modulators without cytotoxic/P-gp induction effects.