Transcriptome analysis and identification of genes associated with bryonolic acid biosynthesis in Trichosanthes cucumerina L.
6
Issued Date
2019
Resource Type
Language
eng
Rights
Mahidol University
Rights Holder(s)
Faculty of Pharmacy Mahidol University
Suggested Citation
Pornpatsorn Lertphadungkit, Somnuk Bunsupa (2019). Transcriptome analysis and identification of genes associated with bryonolic acid biosynthesis in Trichosanthes cucumerina L.. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/49498
Title
Transcriptome analysis and identification of genes associated with bryonolic acid biosynthesis in Trichosanthes cucumerina L.
Author(s)
Other Contributor(s)
Abstract
Trichosanthes cucumerina L. (TC) is a Thai medicinal plant belonging to Cucurbitaceae
family. This plant has been used in Thai traditional medicine for purgative, emetics, treating
dandruff and relieving the sinusitis. Additionally, it has been reported about its activities,
especially cytotoxicity and many studies showed that these activities related with a compound
named bryonolic acid. This compound is one of major triterpenoid compounds that can be
generally found in TC root. This study aimed to improve bryonolic acid content using plant
tissue culture techniques for the study of genes in bryonolic acid biosynthesis. The callus was
induced using Murashige and Skoog (MS) plant media supplemented with 0.5 mg/ml of indole-
3-butyric acid (IBA) and 1 mg/ml of benzylaminopurine (BA). For the cell suspensions, they
were cultured in MS media with 1 mg/ml of IBA and 1 mg/ml of BA. Three elicitors, methyl
jasmonate (50 - 200 μM), yeast extract (0.1 - 2% w/v) and chitosan (1 - 100 mg/ml), were added
into the cell suspension. The results from HPLC analysis showed that callus and cell suspension
presented the higher bryonolic acid levels by just over twenty and seven times compared with
TC root (approximately 2 mg/g dry weight), respectively. In the presence of elicitors, the best
treated condition was to treat cell suspensions with 1 mg/ml of chitosan for 8 days
(approximately 24 mg/g dry weight). To carry out the candidate genes in bryonolic acid
biosynthesis pathway, RNA was extracted from TC calli for the de novo transcriptome analysis
compared with TC leaf and fruit to carry out the candidate genes associated with bryonolic acid
biosynthetic pathway.
Description
The 1st Pharmaceutical Sciences Asia Conference 2019 Theme : Pharmaceutical Sciences toward Health Innovation in the Disruptive Era. Bangkok Midtown Hotel, Thailand. August 22, 2019, page 26-27