Publication: Paper-based immunosensor with competitive assay for cortisol detection
4
Issued Date
2020-01-30
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ISSN
1873264X
07317085
07317085
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2-s2.0-85073709958
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Pharmaceutical and Biomedical Analysis. Vol.178, (2020)
Suggested Citation
Amara Apilux, Sirirat Rengpipat, Wilasinee Suwanjang, Orawon Chailapakul Paper-based immunosensor with competitive assay for cortisol detection. Journal of Pharmaceutical and Biomedical Analysis. Vol.178, (2020). doi:10.1016/j.jpba.2019.112925 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/49539
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Title
Paper-based immunosensor with competitive assay for cortisol detection
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Abstract
© 2019 Elsevier B.V. The measurement of cortisol (stress hormone) is important for diagnosis and monitoring of stress-related diseases. A paper-based immunosensor with a competitive assay was developed for quick and easy detection of cortisol levels in serum. The paper-based sensor was fabricated using a simple and cheap wax printing method. Cortisol conjugated-BSA was immobilized on the paper's surface in the detection zone for the competitive immunoassay. Anti-cortisol mAb-conjugated gold nanoparticles, as signal indicator, were used to detect cortisol in the sample. A 2-step procedure included applying the sample and washing for cortisol determination. The results showed that the device had the ability to differentiate cortisol levels into three ranges: < 25 μg/dL, 25–50 μg/dL and > 50 μg/dL by visual detection. The limit of detection determined by an image processing program was 21.5 μg/dL. This assay was successfully developed to detect cortisol in serum, and showed good recovery and precision. Our results correlated well with those obtained using an electrochemiluminescence method. This paper-based immunosensor provided a rapid and simple screening test for serum cortisol detection.