Publication: Localization of phospholipase C β3 in the major salivary glands of adult mice
2
Issued Date
2019-05-01
Resource Type
ISSN
16180372
00651281
00651281
Other identifier(s)
2-s2.0-85064316252
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Acta Histochemica. Vol.121, No.4 (2019), 484-490
Suggested Citation
Atsara Rawangwong, Atthapon Pidsaya, Wipawee Thoungseabyoun, Apussara Tachow, Tarinee Sawatpanich, Waraporn Sakaew, Miwako Yamasaki, Masahiko Watanabe, Hisatake Kondo, Wiphawi Hipkaeo Localization of phospholipase C β3 in the major salivary glands of adult mice. Acta Histochemica. Vol.121, No.4 (2019), 484-490. doi:10.1016/j.acthis.2019.04.006 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/50192
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Localization of phospholipase C β3 in the major salivary glands of adult mice
Abstract
© 2019 Elsevier GmbH Phospholipase C (PLC)β has a role in saliva secretion by controlling intracellular Ca2+ via its product, IP3. The present study was attempted to localize PLCβ isoforms in mouse salivary glands in situ. A single major band was detected for PLCβ3 in immunoblots of the parotid and sublingual glands (PG, SLG), while no such band was seen in the submandibular gland (SMG). No bands were detected for PLCβ1 or 4 in the three glands. In immuno-light microscopy of PG and SLG, substantial immunoreactivity for PLCβ3 was seen in the cytoplasm including the plasmalemma of almost all ductal cells, while no distinct immunoreactivity was discerned in most acinar cells except for sublingual demilune cells. Numerous ductal cells exhibited higher immunoreactivity for PLCβ3 in their apical/supranuclear cell domain including the plasmalemma than in the basal/infranuclear domain, indicating an apico-basal polarity. In immuno-gold electron microscopy of PG ducts and SLG ducts and demilunes, most gold particles were found in association with plasma membranes as well as various intracellular membranes, most of which formed small oblong or flattened vesicles and vacuoles. A few particles were seen without association with any membranous structures. The present finding supports the previous physio-pharmacological result that Ca2+-signaling proteins as well as initial intracellular Ca2+ changes occur in the apical cell domain including the plasma membranes of the exocrine cells.