Publication: Development of a simple and rapid method for the detection of isomiroestrol in Pueraria candollei by an immunochromatographic strip test
1
Issued Date
2019-06-14
Resource Type
ISSN
18610293
13403443
13403443
Other identifier(s)
2-s2.0-85064213877
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Natural Medicines. Vol.73, No.3 (2019), 577-583
Suggested Citation
Jiranan Chaingam, Tharita Kitisripanya, Supaluk Krittanai, Seiichi Sakamoto, Hiroyuki Tanaka, Waraporn Putalun Development of a simple and rapid method for the detection of isomiroestrol in Pueraria candollei by an immunochromatographic strip test. Journal of Natural Medicines. Vol.73, No.3 (2019), 577-583. doi:10.1007/s11418-019-01307-6 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/50561
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Development of a simple and rapid method for the detection of isomiroestrol in Pueraria candollei by an immunochromatographic strip test
Other Contributor(s)
Abstract
© 2019, The Japanese Society of Pharmacognosy. Pueraria candollei (P. candollei) is a traditional Thai herb widely used for estrogen replacement therapy because it contains many unique chromenes that possess potent estrogenic activity, one of which is known as isomiroestrol. Since isomiroestrol is a promising compound that is solely present in P. candollei, it can be used as an identifying marker for standardization of P. candollei. Here, we developed a lateral-flow immunochromatographic strip (ICS) test using a colloidal gold nanoparticle-conjugated anti-isomiroestrol monoclonal antibody (12C1-mAb) for the detection of isomiroestrol in plant samples and products of P. candollei. The advantages of the developed ICS over an enzyme-linked immunosorbent assay are its simplicity and rapidity, as the ICS test can be completed 15 min after dipping the strip into the analyte solution. The detectable concentration of isomiroestrol was 7.0 µg/mL. Considering the demand for the standardization of P. candollei due to concerns regarding its quality, our ICS test using isomiroestrol as an identifying marker would be effective and useful to assess the presence of isomiroestrol.