Publication: Identification of apigenin and luteolin in Artemisia annua l. for the quality control
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2019-01-01
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22288082
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2-s2.0-85067197770
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Mahidol University
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Siriraj Medical Journal. Vol.71, No.3 (2019), 240-245
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Rattana Phadungrakwittaya, Sirikul Chotewuttakorn, Manoon Piwtong, Onusa Thamsermsang, Tawee Laohapand, Pravit Akarasereenont (2019). Identification of apigenin and luteolin in Artemisia annua l. for the quality control. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/52107.
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Identification of apigenin and luteolin in Artemisia annua l. for the quality control
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Abstract
© 2019, Siriraj Medical Journal. Objective: To identify active compounds and establish the chemical fingerprint of Artemisia annua L. for the quality control. Methods: Thin-layer chromatography (TLC) conditions were developed to screen for 2 common flavonoids (apigenin and luteolin). Three mobile phases were used to isolate these flavonoids in 80% ethanolic extract of A. annua. Hexane: ethyl acetate: acetic acid (31:14:5, v/v) and toluene: 1,4-dioxane: acetic acid (90:25:4, v/v) were used in normal phase TLC (NP-TLC), and 5.5% formic acid in water: methanol (50:50, v/v) were used in reverse phase TLC (RP-TLC). Chromatograms were visualized under visible light after spraying with Fast Blue B Salt. Apigenin and luteolin bands were checked by comparing their Rf values and UV-Vis absorption spectra with reference markers. Results: Apigenin and luteolin were simultaneously detected with good specificity in RP-TLC condition, while only apigenin was detected in NP-TLC condition. Apigenin band intensity was higher than luteolin band intensity in both conditions. Conclusion: This knowledge can be applied to the development of quality control assessments to ensure product efficacy and consistency.