Publication: Novel paper-based colorimetric immunoassay (PCI) for sensitive and specific detection of salbutamol residues in flesh of swine and urine using Ag<inf>3</inf>PO<inf>4</inf>/Ag nanocomposite as label
9
Issued Date
2020-01-01
Resource Type
ISSN
17503841
00221147
00221147
Other identifier(s)
2-s2.0-85076849774
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Food Science. Vol.85, No.1 (2020), 209-219
Suggested Citation
Nutthaporn Malahom, Purim Jarujamrus, Wipark Anutrasakda, Runglawan Chawengkirttikul, Atitaya Siripinyanond, Rattapol Meelapsom, Maliwan Amatatongchai Novel paper-based colorimetric immunoassay (PCI) for sensitive and specific detection of salbutamol residues in flesh of swine and urine using Ag<inf>3</inf>PO<inf>4</inf>/Ag nanocomposite as label. Journal of Food Science. Vol.85, No.1 (2020), 209-219. doi:10.1111/1750-3841.14974 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/53533
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Title
Novel paper-based colorimetric immunoassay (PCI) for sensitive and specific detection of salbutamol residues in flesh of swine and urine using Ag<inf>3</inf>PO<inf>4</inf>/Ag nanocomposite as label
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Abstract
© 2019 Institute of Food Technologists® Abstract: Salbutamol (SAL) can cause potential hazards to human health and its use as a growth promoter in meat-producing animals is illegal. This work reports a novel approach for competitive paper-based colorimetric immunoassay (PCI) using the Ag3PO4/Ag nanocomposite as label for sensitive and specific determination of SAL in flesh of swine and urine. The Ag3PO4/Ag nanocomposite was synthesized by a one-step chemical bath method, which could instantly oxidize a chromogenic substrate for the color development under acidic conditions without the participation of H2O2. This approach provides high affinity between the Ag3PO4/Ag nanocomposite and the substrate (with the Michaelis–Menten constant of 0.44 mM). In addition, the fabrication process of the PCI was simple and cost-effective. Particularly, the novel PCI also exhibits simplicity and cost-effectiveness of the fabrication process through a simple wax screen-printing, which requires inexpensive equipment and material including a screen, wax, a squeegee, and a hair dryer. Under optimal conditions, the competitive PCI exhibited a linearity range of 0.025 to 1.00 µg/L. The developed approach offers advantages over the conventional ELISA for the purpose of routine use because it requires a shorter incubation time (<1 hr), significantly small volumes of reagents and samples (<100 µL each), and an inexpensive consumer-grade digital camera coupled with a simple gray-scale transformation of the RGB (Red Green Blue) color image for the purpose of quantification of the detection. Practical Application: Salbutamol (SAL) can cause potential hazards to human health and the use of which as growth promoter in meat-producing animals is illegal. This work introduces a novel approach for competitive immunoassay on paper-based colorimetric immunoassay using the Ag3PO4/Ag nanocomposite as the label (instead of using natural enzyme) for low-cost, sensitive, and specific determination of SAL residues at low level in flesh of swine and urine samples. The proposed approach offers advantages over the conventional ELISA for the purpose of routine use.