Publication: Caractéristiques à l'échelle du génome des microsatellites de cinq espèces humaines de Plasmodium, spécialement Plasmodium malariae et P. ovale curtisi
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Issued Date
2020-01-01
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ISSN
17761042
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2-s2.0-85084785372
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Mahidol University
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SCOPUS
Bibliographic Citation
Parasite (Paris, France). Vol.27, (2020), 34
Suggested Citation
Vivek Bhakta Mathema, Supatchara Nakeesathit, Nicholas J. White, Arjen M. Dondorp, Mallika Imwong Caractéristiques à l'échelle du génome des microsatellites de cinq espèces humaines de Plasmodium, spécialement Plasmodium malariae et P. ovale curtisi. Parasite (Paris, France). Vol.27, (2020), 34. doi:10.1051/parasite/2020034 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/56097
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Caractéristiques à l'échelle du génome des microsatellites de cinq espèces humaines de Plasmodium, spécialement Plasmodium malariae et P. ovale curtisi
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Abstract
© V.B. Mathema et al., published by EDP Sciences, 2020. Microsatellites can be utilized to explore genotypes, population structure, and other genomic features of eukaryotes. Systematic characterization of microsatellites has not been a focus for several species of Plasmodium, including P. malariae and P. ovale, as the majority of malaria elimination programs are focused on P. falciparum and to a lesser extent P. vivax. Here, five human malaria species (P. falciparum, P. vivax, P. malariae, P. ovale curtisi, and P. knowlesi) were investigated with the aim of conducting in-depth categorization of microsatellites for P. malariae and P. ovale curtisi. Investigation of reference genomes for microsatellites with unit motifs of 1-10 base pairs indicates high diversity among the five Plasmodium species. Plasmodium malariae, with the largest genome size, displays the second highest microsatellite density (1421 No./Mbp; 5% coverage) next to P. falciparum (3634 No./Mbp; 12% coverage). The lowest microsatellite density was observed in P. vivax (773 No./Mbp; 2% coverage). A, AT, and AAT are the most commonly repeated motifs in the Plasmodium species. For P. malariae and P. ovale curtisi, microsatellite-related sequences are observed in approximately 18-29% of coding sequences (CDS). Lysine, asparagine, and glutamic acids are most frequently coded by microsatellite-related CDS. The majority of these CDS could be related to the gene ontology terms "cell parts," "binding," "developmental processes," and "metabolic processes." The present study provides a comprehensive overview of microsatellite distribution and can assist in the planning and development of potentially useful genetic tools for further investigation of P. malariae and P. ovale curtisi epidemiology.