Publication: Proteomic Analysis of the Anoikis-Resistant Human Breast Cancer Cell Lines
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Issued Date
2020-01-01
Resource Type
ISSN
19406029
10643745
10643745
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2-s2.0-85082562019
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Mahidol University
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SCOPUS
Bibliographic Citation
Methods in Molecular Biology. Vol.2138, (2020), 185-193
Suggested Citation
Chareeporn Akekawatchai, Sittiruk Roytrakul, Narumon Phaonakrop, Janthima Jaresitthikunchai, Sarawut Jitrapakdee Proteomic Analysis of the Anoikis-Resistant Human Breast Cancer Cell Lines. Methods in Molecular Biology. Vol.2138, (2020), 185-193. doi:10.1007/978-1-0716-0471-7_11 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/56138
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Title
Proteomic Analysis of the Anoikis-Resistant Human Breast Cancer Cell Lines
Abstract
© 2020, Springer Science+Business Media, LLC, part of Springer Nature. Acquisition of anoikis resistance is a prerequisite for cancer metastasis and invasion, which are major causes of death from cancer. The molecular mechanisms underlying antianoikis properties in cancer cells are still largely unclear. Here, we describe a protocol for preparation of anoikis-resistant cultured nonmetastatic MCF-7 and metastatic MDA-MB-231 cell lines. The anoikis-resistant cultures were prepared by plating cells in the poly-2-hydroxyethyl methacrylate coated plates and cultured for 24 h. The viability of cells in the cultures was determined using trypan blue staining and annexin V cell death assay, while protein profiles associated with anoikis-resistance in both cells and conditioned media were analyzed by proteomics.