Publication: Interepidemic detection of chikungunya virus infection and transmission in Northeastern Thailand
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Issued Date
2020-10-01
Resource Type
ISSN
14761645
00029637
00029637
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2-s2.0-85092707194
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Mahidol University
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SCOPUS
Bibliographic Citation
American Journal of Tropical Medicine and Hygiene. Vol.103, No.4 (2020), 1660-1669
Suggested Citation
Bao Chi Thi Le, Tipaya Ekalaksananan, Kesorn Thaewnongiew, Supranee Phanthanawiboon, Sirinart Aromseree, Thipruethai Phanitchat, Jureeporn Chuerduangphui, Apiporn T. Suwannatrai, Neal Alexander, Hans J. Overgaard, Michael J. Bangs, Chamsai Pientong Interepidemic detection of chikungunya virus infection and transmission in Northeastern Thailand. American Journal of Tropical Medicine and Hygiene. Vol.103, No.4 (2020), 1660-1669. doi:10.4269/ajtmh.20-0293 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/59989
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Title
Interepidemic detection of chikungunya virus infection and transmission in Northeastern Thailand
Abstract
Copyright © 2020 by The American Society of Tropical Medicine and Hygiene Chikungunya fever is a viral mosquito-borne, acute febrile illness associated with rash, joint pain, and occasionally prolonged polyarthritis. Chikungunya outbreaks have been reported worldwide including many provinces of Thailand. Although chikungunya virus (CHIKV) occurs in Thailand, details on its epidemiology are lacking compared with dengue, a common mosquito-borne disease in the country. Therefore, study on CHIKV and its epidemiology in both humans and mosquitoes is required to better understand its importance clinically and dynamics in community settings. So a prospective examination of virus circulation in human and mosquito populations in northeastern Thailand using serological and molecular methods, including the genetic characterization of the virus, was undertaken. The study was conducted among febrile patients in eight district hospitals in northeastern Thailand from June 2016 to October 2017. Using real-time PCR on the conserved region of nonstructural protein 1 gene, CHIKV was detected in eight (4.9%) of 161 plasma samples. Only one strain yielded a sequence of sufficient size allowing for phylogenetic analysis. In addition, anti-CHIKV IgM and IgG were detected in six (3.7%) and 17 (10.6%) patient plasma samples. The single sequenced sample belonged to the East/Central/South Africa (ECSA) genotype and was phylogenetically similar to the Indian Ocean sub-lineage. Adult Aedes mosquitoes were collected indoors and within a 100-m radius from the index case house and four neighboring houses. CHIKV was detected in two of 70 (2.9%) female Aedes aegypti mosquito pools. This study clearly demonstrated the presence and local transmission of the ECSA genotype of CHIKV in the northeastern region of Thailand.