Rapid latex agglutination assays for serodiagnosis of human
1
Issued Date
2013
Resource Type
Language
eng
Rights
Mahidol University
Suggested Citation
Vichaya Suttisunhakul, วิชญะ สุทธิสัณหกุล, Chanthiwa Wikraiphat, จันทร์ทิวา วิกรัยพัฒน์, Burtnick M., Wuthiekanun V., Brett P., Narisara Chantratita, นริศรา จันทราทิตย์ (2013). Rapid latex agglutination assays for serodiagnosis of human. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/63170
Title
Rapid latex agglutination assays for serodiagnosis of human
Abstract
Burkholderia pseudomallei is the etiologic agent of melioidosis, a significant community-acquired and
severe disease in endemic regions. Diagnosis and treatment of human infections caused by this
organism is challenging. In the absence of chemotherapeutic intervention, acute disease is frequently
fatal. The objective of this project is to identify and characterize immunogens expressed by B. pseudomallei
for developing rapid serodiagnostic tests. Recently, we have demonstrated that the O-polysaccharides (OPS)
and capsular polysaccharides (CPS) are suited for this task. We evaluated the use of OPS- and CPS-based latex
agglutination assays as a rapid approach for serodiagnosing human melioidosis. OPS and CPS antigens were
isolated from B. pseudomallei Bp82, using a modified hot aqueous-phenol extraction procedure. Following
purification by sequential rounds of ultracentrifugation and size-exclusion chromatography, the carbohydrate
antigens were chemically activated with sodium periodate and covalently linked to aliphatic amine-derivatized
latex beads via reductive amination to produce two distinct immunoassays. Utilizing rabbit antiserum samples
raised against formalin fixed B. pseudomallei, B. thailandensis and B. cenocepacia isolates, the resulting OPS- and
CPS-coated beads exhibited agglutination patterns consistent with those that would be predicted. Using 156
culture-confirmed human melioidosis patients and 143 healthy donor serum samples, the sensitivity and specificity of OPS-coated beads were 82.1% and 62.9% respectively, and of CPS-coated beads were 65.4 and
69.2% respectively. The performance of these assays was equal to or greater than those associated with indirect
hemagglutination assays (sensitivity 68.0%, specificity 67.1%). Our rapid latex agglutination assays may be
useful as stable, affordable, point-of-care tests for serodiagnosing human melioidosis.
Description
Joint International Tropical Medicine Meeting 2013: Towards global health: an Asian paradigm of Tropical Medicine 11-13 December 2013 Centara Grand Bangkok Convention Center at Central World, Bangkok,Thailand. Bangkok: Faculty of Tropical Medicine, Mahidol University; 2013. p.156-157.