Modified thawing process yielded a high number of viable-intact erythrocytic stages of the longterm cryopreserved plasmodium vivax malaria
4
Issued Date
2014
Resource Type
Language
eng
Rights
Mahidol University
Suggested Citation
Sangdao Somsri, Titipatima Sakulterdkiat, Mathirut Mungthin, Naruemon Sitthichot, Poom Adisakwattana, ภูมิ อดิศักดิ์วัฒนา, Rachanee Udomsangpetch (2014). Modified thawing process yielded a high number of viable-intact erythrocytic stages of the longterm cryopreserved plasmodium vivax malaria. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/63171
Title
Modified thawing process yielded a high number of viable-intact erythrocytic stages of the longterm cryopreserved plasmodium vivax malaria
Other Contributor(s)
Abstract
Cryopreservation is an important technique for long-term starage of malaria
for use in the laboratory as well as preservation of field isolates. For decades,
freezing medium containing glycerolyte has been the solution of choice for
preservation of the parasites. The current technique available for the preservation of
P. falciparum and P. vivax demonstrate successful cryopreservation of parasites in the
early stages of the life cycle from both cultureadapted parasites and infected blood
samples taken directly from the host. However, parasites in mature stages cannot be
preserved by the standard thawing technique. Low recovery of parasites following the
freezing and thawing process may be a selective event favoring mutant subpopulation;
therefore, it is essential that the preservation technique used is capable of preserving
parasites at all stages of its life cycle. In this study, we introduce a modified method of
malaria parasite cryopreservation that results in significantly higher parasite recovery
of all stages (p<0.001). Higher percentage of mature parasites was recovered from
both malarial spices of P. falciparum and P. vivax, when compared the modified
thawing method to the standard method (p<0.001). This modified thawing method will
enhance opportunity for research in P. vivax strain allowing successful maintenance of
long-term in vitro culture, assessment of drug effectiveness and vaccine development
studies.
Description
Joint International Tropical Medicine Meeting 2014: 3D perspectives on tropical medicine: drivers, diversity and determination the 8th seminar on food-and water-borne parasitic zoonoses: 2-4 December 2014: Centara Grand Bangkok Convention Center at Central World, Bangkok, Thailand. Bangkok: Faculty of Tropical Medicine, Mahidol University; 2014. p. 276.