Mass spectrometric analysis of particular proteins of thai paragonimus heterotremus worms
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2014
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Mahidol University
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Kanokkarn Pothong, กนกกาญจน์ โพธิ์ทอง, Paron Dekumyoy, พารณ ดีคำย้อย, Thareerat Kalambaheti, ธารีรัตน์ กะลัมพะเหติ, Dorn Watthanakulpanich, ดร วัฒนกุลพานิชย์, Chalit Komalmisra, ชลิต โกมลมิศร์, Timothy P Yoshino (2014). Mass spectrometric analysis of particular proteins of thai paragonimus heterotremus worms. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/63339.
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Mass spectrometric analysis of particular proteins of thai paragonimus heterotremus worms
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Abstract
Paragonimus heterotremus is the causative agent of human paragonimiasis in
Thailand. The protein molecular weights (MW) of 32.5, 33 and 35 kDa were
used as diagnosis bands, and were reacted with human paragonimiasis
sera confirmed by the presence of eggs and parasites, for immunoblotting. To study
biological systems of the immunodominant worm antigen, protein MWs at 25-50 kDa
were analyzed by Mass Spectrometry, Scaffold Version 4 and Blast2go against NCBI.
The Top-Hit species were Clonorchissinensis, Schistosoma japonicum, Schistosoma
mansoni, Paragonimus westermani, Fasciola hepatica and other parasites. The
sequence distribution of molecular function indicated that most functions were binding,
catalytic activity or ion binding. Mass Spectrometry data showed that proteins at
32.5, 33 and 35 kDa were succinate dehydrogenase complex, subunit B, iron sulfur
[Schistosoma japonicum], actin beta/gamma 1 [Clonorchis sinensis] and tyrosine
3-monooxygenase/tryptophan 5-monooxygenase activation protein [Clonorchis
sinensis]. This study aimed to understand more about the antigenic proteins of P.
heterotremus in terms of protein identification, functions and their components. This
data will also be used to develop and produce new types of antigen for immunodiagnostic
applications.
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Joint International Tropical Medicine Meeting 2014: 3D perspectives on tropical medicine: drivers, diversity and determination the 8th seminar on food-and water-borne parasitic zoonoses: 2-4 December 2014: Centara Grand Bangkok Convention Center at Central World, Bangkok, Thailand. Bangkok: Faculty of Tropical Medicine, Mahidol University; 2014. p. 277.