Publication: Establishment of hematopoietic tissue primary cell cultures from the giant freshwater prawn Macrobrachium rosenbergii
2
Issued Date
2021-04-01
Resource Type
ISSN
15730778
09209069
09209069
Other identifier(s)
2-s2.0-85100185730
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Mahidol University
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SCOPUS
Bibliographic Citation
Cytotechnology. Vol.73, No.2 (2021), 141-157
Suggested Citation
Kwanta Thansa, Thanapong Kruangkum, Arnon Pudgerd, Lamai Chaichandee, Piti Amparyup, Rungkarn Suebsing, Charoonroj Chotwiwatthanakun, Rapeepun Vanichviriyakit, Kallaya Sritunyalucksana Establishment of hematopoietic tissue primary cell cultures from the giant freshwater prawn Macrobrachium rosenbergii. Cytotechnology. Vol.73, No.2 (2021), 141-157. doi:10.1007/s10616-021-00451-w Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/76235
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Title
Establishment of hematopoietic tissue primary cell cultures from the giant freshwater prawn Macrobrachium rosenbergii
Abstract
The giant freshwater prawn Macrobrachium rosenbergii is one of the most important aquaculture species in Southeast Asia. In this study, in vitro culture of its hematopoietic tissue cells was achieved and characterized for use as a tool to study its pathogens that cause major farm losses. By transmission electron microscopy, the ultrastructure of the primary culture cells was similar to that of cells lining intact hematopoietic tissue lobes. Proliferating cell nuclear antigen (PCNA) (a marker for hematopoietic stem cell proliferation) was detected in some of the cultured cells by polymerase chain reaction (PCR) testing and flow cytometry. Using a specific staining method to detect phenoloxidase activity and using PCR to detect expression markers for semigranular and granular hemocytes (e.g., prophenoloxidase activating enzyme and prophenoloxidase) revealed that some of the primary cells were able to differentiate into mature hemocytes within 24 h. These results showed that some cells in the cultures were hematopoietic stem cells that could be used to study other interesting research topics (e.g. host pathogen interactions and development of an immortal hematopoietic stem cell line).