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A potent neutralizing mouse monoclonal antibody specific to dengue virus type 1 Mochizuki strain recognized a novel epitope around the N-67 glycan on the envelope protein: A possible explanation of dengue virus evolution regarding the acquisition of N-67 glycan

4

Issued Date

2021-03-01

Resource Type

Article

ISSN

18727492
01681702

DOI

10.1016/j.virusres.2020.198278

Other identifier(s)

2-s2.0-85099142601

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Virus Research. Vol.294, (2021)

Suggested Citation

Tomohiro Kotaki, Atsushi Yamanaka, Eiji Konishi, Masanori Kameoka A potent neutralizing mouse monoclonal antibody specific to dengue virus type 1 Mochizuki strain recognized a novel epitope around the N-67 glycan on the envelope protein: A possible explanation of dengue virus evolution regarding the acquisition of N-67 glycan. Virus Research. Vol.294, (2021). doi:10.1016/j.virusres.2020.198278 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/76273

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Title

A potent neutralizing mouse monoclonal antibody specific to dengue virus type 1 Mochizuki strain recognized a novel epitope around the N-67 glycan on the envelope protein: A possible explanation of dengue virus evolution regarding the acquisition of N-67 glycan

Author(s)

Tomohiro Kotaki
 Atsushi Yamanaka
 Eiji Konishi
 Masanori Kameoka

Other Contributor(s)

Research Institute for Microbial Diseases
 Mahidol University
 Kobe University

Abstract

The analysis of neutralizing epitope of dengue virus (DENV) is important for the development of an effective dengue vaccine. A potent neutralizing mouse monoclonal antibody named 7F4 was previously reported and, here, we further analyzed the detailed epitope of this antibody. 7F4 recognized a novel conformational epitope close to the N-67 glycan on the envelope protein. This antibody was specific to the DENV that lacks N-67 glycan, including the Mochizuki strain. Interestingly, the Mochizuki strain acquired N-67 glycan by 7F4 selective pressure. Considering that most of the currently circulating DENVs possess N-67 glycan, DENVs may have evolved to escape from antibodies targeting 7F4 epitope, suggesting the potency of this neutralizing epitope. In addition, this study demonstrated the existence of the epitopes close to 7F4 epitope and their crucial role in neutralization. In conclusion, the epitopes close to the N-67 glycan are attractive targets for the dengue vaccine antigen. Further analysis of this epitope is warranted.

Keyword(s)

Biochemistry, Genetics and Molecular Biology
 Immunology and Microbiology
 Medicine

Availability

URI

https://repository.li.mahidol.ac.th/handle/123456789/76273

Collections

Scopus 2021