Publication: Susceptibility of Anopheles sinensis to Plasmodium vivax in malarial outbreak areas of central China.
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Accepted Date
2013-06-10
Issued Date
2013-06-14
Copyright Date
2013
Resource Type
Language
eng
ISSN
1756-3305 (electronic)
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Mahidol University
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BioMed Central
Bibliographic Citation
Zhu G, Xia H, Zhou H, Li J, Lu F, Liu Y. et al. Susceptibility of Anopheles sinensis to Plasmodium vivax in malarial outbreak areas of central China. Parasit Vectors. 2013 Jun 14;6:176.
Suggested Citation
Zhu, Guoding, Xia, Hui, Zhou, Huayun, Li, Julin, Lu, Feng, Liu, Yaobao, Cao, Jun, Gao, Qi, Jetsumon Sattabongkot, เจตสุมน สัตตบงกช Susceptibility of Anopheles sinensis to Plasmodium vivax in malarial outbreak areas of central China.. Zhu G, Xia H, Zhou H, Li J, Lu F, Liu Y. et al. Susceptibility of Anopheles sinensis to Plasmodium vivax in malarial outbreak areas of central China. Parasit Vectors. 2013 Jun 14;6:176.. doi:10.1186/1756-3305-6-176. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/763
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Title
Susceptibility of Anopheles sinensis to Plasmodium vivax in malarial outbreak areas of central China.
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Abstract
BACKGROUND: Anopheles sinensis, Anopheles anthropophagus, Anopheles minimus and
Anopheles dirus are the major vectors of malaria transmission in China. Anopheles
sinensis is considered a secondary vector due to its relatively low
malaria-transmission ability. However, in 2005, an outbreak of over 40,000
Plasmodium vivax malaria cases was reported in areas where Anopheles sinensis was
the only major vector. Therefore, it is necessary to reassess the malaria
transmission ability of this vector species in China.
METHODS: Laboratory colonies of An. sinensis and An. anthropophagus, and
first-generation progeny (F1) of An. sinensis that had been collected in central
China, were infected by direct membrane feeding assay with mono-vivax
gametocyte-containing blood collected from vivax-infected patients. The
mosquitoes were kept for 7 to 14 days post-blood feeding to allow parasites to
develop into oocysts and sporozoites. Infectivity was measured by dissecting
midguts and salivary glands. The presence of oocysts and sporozoites was
determined by microscopy at 7 and 14 days post-blood feeding, and the numbers of
gametocytes and asexual parasites, as well as mosquito parasite infections, were
determined.
RESULTS: The positive oocyst and sporozoite feed rates of the 142 pairs of
lab-colony An. sinensis and An. anthropophagus were not significantly different,
and the same results were found with the 10 pairs of laboratory and F1 An.
sinensis. An. sinensis had more oocysts/midgut at 7 days post-feeding than An.
anthropophagus, but the gametocytemia, asexual parasitemia, and ratio of
macrogametocytes to microgametocytes, did not correlate with either oocyst or
sporozoite infection. However, in the oocyst-positive mosquitoes, there was a
correlation between gametocytemia and the average oocyst number/midgut.
CONCLUSIONS: The susceptibility of An. sinensis (both laboratory and F1) to P.
vivax-infected blood is similar to Anopheles anthropophagus, when evaluated by
membrane feeding assay under laboratory conditions. In recent years, in central
China, the vivax malaria transmission ability of An. sinensis has probably been
underestimated. Further studies of this species in other regions are needed. An.
sinensis could also be a good candidate vector for evaluating candidate malaria
transmission-blocking vaccines (TBV).