Publication: Use of Bacterial Luciferase as a Reporter Gene in Eukaryotic Systems
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Issued Date
2021-01-01
Resource Type
ISSN
19406029
10643745
10643745
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2-s2.0-85107028302
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Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Methods in Molecular Biology. Vol.2274, (2021), 53-65
Suggested Citation
Jittima Phonbuppha, Ruchanok Tinikul, Pimchai Chaiyen Use of Bacterial Luciferase as a Reporter Gene in Eukaryotic Systems. Methods in Molecular Biology. Vol.2274, (2021), 53-65. doi:10.1007/978-1-0716-1258-3_6 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/76371
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Title
Use of Bacterial Luciferase as a Reporter Gene in Eukaryotic Systems
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Abstract
Reporter gene assays are powerful tools for monitoring dynamic molecular changes and for evaluating the responses that occur at the genetic elements within cells in response to exogenous molecules. In general, various protein systems can be used as reporter genes, including luciferases. Here, the present protocol introduces a unique reporter gene system for monitoring molecular events in cells using bacterial luciferase (lux), which can generate blue-green light suitable for gene reporter applications with the highest cost performance. The protocol also guides the assay conditions and necessary components for using of lux gene (lux) as a eukaryotic reporter system. The lux system can be applied to monitor variety of molecular events inside mammalian cellular systems.