Publication: Validation of RNA Aptamer Probes to Image Candida albicans in Paraffin-Embedded Sections of Wistar Rat Tongue
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Issued Date
2021-01-01
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ISSN
13057464
13057456
13057456
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2-s2.0-85118529457
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Mahidol University
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SCOPUS
Bibliographic Citation
European Journal of Dentistry. (2021)
Suggested Citation
Boy M. Bachtiar, Chatchawan Srisawat, Retno Pudji Rahayu, Retno D. Soedjodono, Silvia Arin Prabandari, Endang W. Bachtiar Validation of RNA Aptamer Probes to Image Candida albicans in Paraffin-Embedded Sections of Wistar Rat Tongue. European Journal of Dentistry. (2021). doi:10.1055/s-0041-1735794 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/76818
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Title
Validation of RNA Aptamer Probes to Image Candida albicans in Paraffin-Embedded Sections of Wistar Rat Tongue
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Abstract
Objective ?This study aimed to validate the use of Ca-apt-1, an RNA aptamer, that we generated previously as a probe for immunostaining of Candida albicans in rat tongue paraffin-fixed tissue sections Material and Methods ?The performance of Ca-apt-1 as a detector molecule was compared with that of anti- C. albicans polyclonal antibody (PcAb), which was used as a positive control. Immunostaining images were visualized by light microscopy and were analyzed by using ImageJ software. Results ?Microscopic results demonstrated that Ca-apt-1 specifically recognized and immunostained C. albicans cells of rat tongue candidiasis, with a specificity comparable to that of PcAb. ImageJ analysis showed that the area (pixels) detected by Ca-apt-1 was wider than that detected by the antibody. This indicates that the binding affinity of Ca-apt-1 toward C. albicans was better than that of PcAb on paraffin-embedded tissues. Conclusion ?This study demonstrates that Ca-apt-1 can be used as a probe for immunostaining of fixed tissue sections for oral candidiasis diagnosis.