Publication: The expansion of activated naive DNA autoreactive B cells and its association with disease activity in systemic lupus erythematosus patients
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Issued Date
2021-12-01
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ISSN
14786362
14786354
14786354
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2-s2.0-85110999600
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Mahidol University
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SCOPUS
Bibliographic Citation
Arthritis Research and Therapy. Vol.23, No.1 (2021)
Suggested Citation
Kittikorn Wangriatisak, Chokchai Thanadetsuntorn, Thamonwan Krittayapoositpot, Chaniya Leepiyasakulchai, Thanitta Suangtamai, Pintip Ngamjanyaporn, Ladawan Khowawisetsut, Prasong Khaenam, Chavachol Setthaudom, Prapaporn Pisitkun, Patchanee Chootong The expansion of activated naive DNA autoreactive B cells and its association with disease activity in systemic lupus erythematosus patients. Arthritis Research and Therapy. Vol.23, No.1 (2021). doi:10.1186/s13075-021-02557-0 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/77159
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Title
The expansion of activated naive DNA autoreactive B cells and its association with disease activity in systemic lupus erythematosus patients
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Abstract
Background: Autoreactive B cells are well recognized as key participants in the pathogenesis of systemic lupus erythematosus (SLE). However, elucidating the particular subset of B cells in producing anti-dsDNA antibodies is limited due to their B cell heterogeneity. This study aimed to identify peripheral B cell subpopulations that display autoreactivity to DNA and contribute to lupus pathogenesis. Methods: Flow cytometry was used to detect total B cell subsets (n = 20) and DNA autoreactive B cells (n = 15) in SLE patients’ peripheral blood. Clinical disease activities were assessed in SLE patients using modified SLEDAI-2 K and used for correlation analyses with expanded B cell subsets and DNA autoreactive B cells. Results: The increases of circulating double negative 2 (DN2) and activated naïve (aNAV) B cells were significantly observed in SLE patients. Expanded B cell subsets and DNA autoreactive B cells represented a high proportion of aNAV B cells with overexpression of CD69 and CD86. The frequencies of aNAV B cells in total B cell populations were significantly correlated with modified SLEDAI-2 K scores. Further analysis showed that expansion of aNAV DNA autoreactive B cells was more related to disease activity and serum anti-dsDNA antibody levels than to total aNAV B cells. Conclusion: Our study demonstrated an expansion of aNAV B cells in SLE patients. The association between the frequency of aNAV B cells and disease activity patients suggested that these expanded B cells may play a role in SLE pathogenesis.