Publication: Ya-hom, a Thai herbal remedy, has opposing effects on histaminergic and cholinergically mediated acid secretory related responses in gastric glands isolated from rabbits
1
Issued Date
2021-01-01
Resource Type
ISSN
25868470
25868195
25868195
Other identifier(s)
2-s2.0-85119922425
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Pharmaceutical Sciences Asia. Vol.48, No.6 (2021), 535-548
Suggested Citation
Wisuda Suvitayavat, Hai Yen Qin, Suwan Thirawarapan, Nuntavan Bunyapraphatsara, Catherine S. Chew Ya-hom, a Thai herbal remedy, has opposing effects on histaminergic and cholinergically mediated acid secretory related responses in gastric glands isolated from rabbits. Pharmaceutical Sciences Asia. Vol.48, No.6 (2021), 535-548. doi:10.29090/psa.2021.06.21.032 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/78555
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Ya-hom, a Thai herbal remedy, has opposing effects on histaminergic and cholinergically mediated acid secretory related responses in gastric glands isolated from rabbits
Other Contributor(s)
Abstract
Ya-hom, a Thai traditional medicine, is used as a treatment for gastric discomfort. We previously found that Ya-hom inhibits both histamine and carbachol-stimulated acid secretion in gastric fistula rats. Herein, we used isolated gastric glands and highly enriched parietal cells from rabbit to determine whether or not Ya-hom acts directly on the acid-secreting parietal cell. Ya-hom inhibited carbachol-stimulated [14C] aminopyrine (AP) accumulation in rabbit gastric glands and prevented carbachol-induced elevation of [Ca2+]i in parietal cells. In contrast, Ya-hom potentiated histamine-stimulated AP accumulation and increased the stimulatory effect of the specific adenylyl cyclase activator, forskolin, in the presence of the histamine H2 receptor antagonist, cimetidine. Ya-hom had no effect on downstream cAMP-dependent signaling events including phosphorylation of LASP1 (LIM and SH3 domain protein 1), and F-actin redistribution. Because the cholinergic signaling pathway is mediated via calcium-dependent mechanisms, we compared effects of Ya-hom on carbachol-induced elevation of [Ca2+]i with those of thapsigargin, a specific endoplasmic reticulum Ca2+ pump inhibitor that also potentiates histamine-stimulated AP accumulation. Both agents similarly potentiated histamine-stimulated AP accumulation and their actions were not additive. Furthermore, Ya-hom inhibited thapsigargin-induced elevation of [Ca2+]i. Based on these findings, we postulate that Ya-hom induces a slow release of calcium from intracellular stores thereby suppressing the cholinergic response and potentiating the cAMP signaling pathway linked to the histamine response. Since Ya-hom directly inhibits the stimulatory effect of carbachol on acid secretion, the gastric action of this herbal remedy may target vagally-dependent HCl secretion.