Publication: Toxicity and Anti-Oxidation Capacity of the Extracts from Caulerpa Lentillifera
7
Issued Date
2021-07-01
Resource Type
ISSN
16851994
Other identifier(s)
2-s2.0-85108789947
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Chiang Mai University Journal of Natural Sciences. Vol.20, No.3 (2021), 1-13
Suggested Citation
Supawadee Osotprasit, Tepparit Samrit, Athit Chaiwichien, Narin Changklungmoa, Krai Meemon, Nakorn Niamnont, Preeyanuch Manohong, Kunwadee Noonong, Montakan Tamtin, Prasert Sobhon, Pornanan Kueakhai Toxicity and Anti-Oxidation Capacity of the Extracts from Caulerpa Lentillifera. Chiang Mai University Journal of Natural Sciences. Vol.20, No.3 (2021), 1-13. doi:10.12982/CMUJNS.2021.065 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/79344
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Toxicity and Anti-Oxidation Capacity of the Extracts from Caulerpa Lentillifera
Abstract
Caulerpa lentillifera (sea grape) has been widely used in pharmaceutical industry and health-care products in Thailand. In this study, we attempted to evaluate the toxicity and antioxidant capacity of sea grape extracts in five fractions (ethanol- CLET, hexane- CLHE, ethyl acetate- CLEA, butanol-CLBU, and aqueous-CLAQ). The extracts were evaluated for cytotoxicity by MTT and LDH assays on four cell lines, fibroblast (L929), macrophages (RAW 264.7), hepatocytes (FL83B), and keratinocytes (HaCaT). Genotoxicity was tested by comet assay and micronucleus assay on human lymphoblast cells (TK6). The antioxidant capacity was measured by DPPH and ABTS scavenging assays. Our results demonstrated low cytotoxicity and genotoxicity of CLET, CLBU and CLAQ. When tested by DPPH and ABTS assays, CLET, CLEA, and CLHE showed high antioxidant activity. In conclusion, CLET, CLBU, and CLAQ demonstrated no toxic effects, and CLET, CLEA, and CLHE exhibited high antioxidant capacity. Therefore, our results indicated that CLET, CLEA, and CLHE could be consumed safely at doses lower than 500 and 200 μg/ml for CLHE and CLEA, respectively.