Publication: An integrated lab-on-chip for rapid identification and simultaneous differentiation of tropical pathogens.
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Accepted Date
2014-06-10
Issued Date
2014-07-31
Copyright Date
2014
Resource Type
Language
eng
ISSN
1935-2727 (printed)
1935-2735 (electronic)
1935-2735 (electronic)
Rights Holder(s)
Public Library of Science
Bibliographic Citation
Tan JJ, Capozzoli M, Sato M, Watthanaworawit W, Ling CL, Mauduit M. et al. An integrated lab-on-chip for rapid identification and simultaneous differentiation of tropical pathogens. PLoS Negl Trop Dis. 2014 Jul 31;8(7):e3043.
Suggested Citation
Tan, Jeslin J. L., Capozzoli, Monica, Sato, Mitsuharu, Wanitda Watthanaworawit, วนิตดา วัฒนวรวิทย์, Ling, Clare L., Mauduit, Marjorie, Malleret, Benoît, Grüner, Anne-Charlotte, Tan, Rosemary, Nosten, François H., Snounou, Georges, Rénia, Laurent, Ng, Lisa F. P. An integrated lab-on-chip for rapid identification and simultaneous differentiation of tropical pathogens.. Tan JJ, Capozzoli M, Sato M, Watthanaworawit W, Ling CL, Mauduit M. et al. An integrated lab-on-chip for rapid identification and simultaneous differentiation of tropical pathogens. PLoS Negl Trop Dis. 2014 Jul 31;8(7):e3043.. doi:10.1371/journal.pntd.0003043 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/798
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Title
An integrated lab-on-chip for rapid identification and simultaneous differentiation of tropical pathogens.
Corresponding Author(s)
Abstract
Tropical pathogens often cause febrile illnesses in humans and are responsible
for considerable morbidity and mortality. The similarities in clinical symptoms
provoked by these pathogens make diagnosis difficult. Thus, early, rapid and
accurate diagnosis will be crucial in patient management and in the control of
these diseases. In this study, a microfluidic lab-on-chip integrating multiplex
molecular amplification and DNA microarray hybridization was developed for
simultaneous detection and species differentiation of 26 globally important
tropical pathogens. The analytical performance of the lab-on-chip for each
pathogen ranged from 102 to 103 DNA or RNA copies. Assay performance was further
verified with human whole blood spiked with Plasmodium falciparum and Chikungunya
virus that yielded a range of detection from 200 to 4×105 parasites, and from 250
to 4×107 PFU respectively. This lab-on-chip was subsequently assessed and
evaluated using 170 retrospective patient specimens in Singapore and Thailand.
The lab-on-chip had a detection sensitivity of 83.1% and a specificity of 100%
for P. falciparum; a sensitivity of 91.3% and a specificity of 99.3% for P.
vivax; a positive 90.0% agreement and a specificity of 100% for Chikungunya
virus; and a positive 85.0% agreement and a specificity of 100% for Dengue virus
serotype 3 with reference methods conducted on the samples. Results suggested the
practicality of an amplification microarray-based approach in a field setting for
high-throughput detection and identification of tropical pathogens.