Kaempferia parviflora Extracts Protect Neural Stem Cells from Amyloid Peptide-Mediated Inflammation in Co-Culture Model with Microglia
3
Issued Date
2023-03-01
Resource Type
eISSN
20726643
Scopus ID
2-s2.0-85149781413
Pubmed ID
36904098
Journal Title
Nutrients
Volume
15
Issue
5
Rights Holder(s)
SCOPUS
Bibliographic Citation
Nutrients Vol.15 No.5 (2023)
Suggested Citation
Temviriyanukul P., Chansawhang A., Karinchai J., Phochantachinda S., Buranasinsup S., Inthachat W., Pitchakarn P., Chantong B. Kaempferia parviflora Extracts Protect Neural Stem Cells from Amyloid Peptide-Mediated Inflammation in Co-Culture Model with Microglia. Nutrients Vol.15 No.5 (2023). doi:10.3390/nu15051098 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/81450
Title
Kaempferia parviflora Extracts Protect Neural Stem Cells from Amyloid Peptide-Mediated Inflammation in Co-Culture Model with Microglia
Author's Affiliation
Other Contributor(s)
Abstract
The existence of neuroinflammation and oxidative stress surrounding amyloid beta (Aβ) plaques, a hallmark of Alzheimer’s disease (AD), has been demonstrated and may result in the activation of neuronal death and inhibition of neurogenesis. Therefore, dysregulation of neuroinflammation and oxidative stress is one possible therapeutic target for AD. Kaempferia parviflora Wall. ex Baker (KP), a member of the Zingiberaceae family, possesses health-promoting benefits including anti-oxidative stress and anti-inflammation in vitro and in vivo with a high level of safety; however, the role of KP in suppressing Aβ-mediated neuroinflammation and neuronal differentiation has not yet been investigated. The neuroprotective effects of KP extract against Aβ42 have been examined in both monoculture and co-culture systems of mouse neuroectodermal (NE-4C) stem cells and BV-2 microglia cells. Our results showed that fractions of KP extract containing 5,7-dimethoxyflavone, 5,7,4′-trimethoxyflavone, and 3,5,7,3′,4′-pentamethoxyflavone protected neural stem cells (both undifferentiated and differentiated) and microglia activation from Aβ42-induced neuroinflammation and oxidative stress in both monoculture and co-culture system of microglia and neuronal stem cells. Interestingly, KP extracts also prevented Aβ42-suppressed neurogenesis, possibly due to the contained methoxyflavone derivatives. Our data indicated the promising role of KP in treating AD through the suppression of neuroinflammation and oxidative stress induced by Aβ peptides.