In vitro transfection efficiencies of T-shaped spermine-based cationic lipids with identical and nonidentical tails under high serum conditions
3
Issued Date
2023-02-03
Resource Type
ISSN
14770520
Scopus ID
2-s2.0-85148670316
Pubmed ID
36762533
Journal Title
Organic and Biomolecular Chemistry
Volume
21
Issue
9
Start Page
1967
End Page
1979
Rights Holder(s)
SCOPUS
Bibliographic Citation
Organic and Biomolecular Chemistry Vol.21 No.9 (2023) , 1967-1979
Suggested Citation
Thongbamrer C., Teerakantrakorn P., Nongpong U., Apiratikul N., Roobsoong W., Kunkeaw N., Nguitragool W., Sattabongkot J., Yingyongnarongkul B.E. In vitro transfection efficiencies of T-shaped spermine-based cationic lipids with identical and nonidentical tails under high serum conditions. Organic and Biomolecular Chemistry Vol.21 No.9 (2023) , 1967-1979. 1979. doi:10.1039/d2ob02129c Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/81654
Title
In vitro transfection efficiencies of T-shaped spermine-based cationic lipids with identical and nonidentical tails under high serum conditions
Other Contributor(s)
Abstract
T-shaped spermine-based cationic lipids with identical and nonidentical hydrophobic tails having variable carbon lengths (from C10 to C18) were designed and synthesized. These lipids were characterized, and their structure-activity relationships were determined for DNA binding and transfection ability of these compounds when formulated as cationic liposomes. These liposomes were then applied as non-viral vectors to transfect HEK293T, HeLa, PC3, H460, HepG2, and Calu′3 cell lines with plasmid DNA encoding the green fluorescent protein. ST9, ST12 and ST13 with nonidentical tails could deliver DNA into HEK293T cells up to 60% under serum-free conditions. The lipid ST15 bearing nonidentical tails was found to be a potent gene transfer agent under 40% serum conditions in HEK293T and HeLa cells. Besides their low cytotoxicity, these lipoplexes also exhibited greater transfection efficiency than the commercially available transfection agent, Lipofectamine 3000.