Nano-Delivery System of Ethanolic Extract of Propolis Targeting Mycobacterium tuberculosis via Aptamer-Modified-Niosomes
Issued Date
2023-01-01
Resource Type
eISSN
20794991
Scopus ID
2-s2.0-85146774791
Journal Title
Nanomaterials
Volume
13
Issue
2
Rights Holder(s)
SCOPUS
Bibliographic Citation
Nanomaterials Vol.13 No.2 (2023)
Suggested Citation
Sangboonruang S., Semakul N., Suriyaprom S., Kitidee K., Khantipongse J., Intorasoot S., Tharinjaroen C.S., Wattananandkul U., Butr-Indr B., Phunpae P., Tragoolpua K. Nano-Delivery System of Ethanolic Extract of Propolis Targeting Mycobacterium tuberculosis via Aptamer-Modified-Niosomes. Nanomaterials Vol.13 No.2 (2023). doi:10.3390/nano13020269 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/81727
Title
Nano-Delivery System of Ethanolic Extract of Propolis Targeting Mycobacterium tuberculosis via Aptamer-Modified-Niosomes
Author's Affiliation
Other Contributor(s)
Abstract
Tuberculosis (TB) therapy requires long-course multidrug regimens leading to the emergence of drug-resistant TB and increased public health burden worldwide. As the treatment strategy is more challenging, seeking a potent non-antibiotic agent has been raised. Propolis serve as a natural source of bioactive molecules. It has been evidenced to eliminate various microbial pathogens including Mycobacterium tuberculosis (Mtb). In this study, we fabricated the niosome-based drug delivery platform for ethanolic extract of propolis (EEP) using thin film hydration method with Ag85A aptamer surface modification (Apt-PEGNio/EEP) to target Mtb. Physicochemical characterization of PEGNio/EEP indicated approximately −20 mV of zeta potential, 180 nm of spherical nanoparticles, 80% of entrapment efficiency, and the sustained release profile. The Apt-PEGNio/EEP and PEGNio/EEP showed no difference in these characteristics. The chemical composition in the nanostructure was confirmed by Fourier transform infrared spectrometry. Apt-PEGNio/EEP showed specific binding to Mycobacterium expressing Ag85 membrane-bound protein by confocal laser scanning microscope. It strongly inhibited Mtb in vitro and exhibited non-toxicity on alveolar macrophages. These findings indicate that the Apt-PEGNio/EEP acts as an antimycobacterial nanoparticle and might be a promising innovative targeted treatment. Further application of this smart nano-delivery system will lead to effective TB management.