Identification of Pathogenic and Opportunistic Yeasts in Pigeon Excreta by MALDI-TOF Mass Spectrometry and Their Prevalence in Chon Buri Province, Thailand
1
Issued Date
2023-02-01
Resource Type
ISSN
16617827
eISSN
16604601
Scopus ID
2-s2.0-85148963772
Pubmed ID
36833884
Journal Title
International Journal of Environmental Research and Public Health
Volume
20
Issue
4
Rights Holder(s)
SCOPUS
Bibliographic Citation
International Journal of Environmental Research and Public Health Vol.20 No.4 (2023)
Suggested Citation
Nualmalang R., Thanomsridetchai N., Teethaisong Y., Sukphopetch P., Tangwattanachuleeporn M. Identification of Pathogenic and Opportunistic Yeasts in Pigeon Excreta by MALDI-TOF Mass Spectrometry and Their Prevalence in Chon Buri Province, Thailand. International Journal of Environmental Research and Public Health Vol.20 No.4 (2023). doi:10.3390/ijerph20043191 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/81872
Title
Identification of Pathogenic and Opportunistic Yeasts in Pigeon Excreta by MALDI-TOF Mass Spectrometry and Their Prevalence in Chon Buri Province, Thailand
Author's Affiliation
Other Contributor(s)
Abstract
Pigeon excreta can cause environmental and public health issues, particularly in urban and public areas. They are reservoirs of several human pathogens including fungi, bacteria, and viruses. Epidemiological data of pathogenic and opportunistic yeasts in pigeon droppings in Chon Buri, one of the most reputable tourist cities of Thailand, are scarce. The present study aimed to identify yeasts in pigeon droppings by MALDI-TOF mass spectrometry, and to study their prevalence in Chon Buri, Thailand. A total of 200 pigeon fecal samples were collected randomly from all 11 districts of Chon Buri. A sum of 393 yeast-like colonies were isolated on Sabourand’s dextrose agar and CHROMagar media. These isolates were further confirmed for their species by MALDI-TOF MS. Twenty-four yeast species belonging to 11 different genera were identified in pigeon fecal samples. Candida spp., predominantly C. krusei (14.32%), were the most prevalent yeast species. Other yeast species, including C. glabrata (12.73%), C. metapsilosis (11.93%), Lodderomyces elongisporus (10.87%), C. tropicalis (7.16%), C. albicans (5.83%), and Cryptococcus neoformans (4.77%) were identified. This study provides valuable epidemiological data and diversity of yeasts in pigeon droppings in Chon Buri, Thailand, and also supports the use of MALDI-TOF MS for yeast identification and epidemiological surveillance.