Different protective capability of chlorogenic acid and quercetin against indomethacin-induced gastrointestinal ulceration
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Issued Date
2023-03-12
Resource Type
eISSN
20427158
Scopus ID
2-s2.0-85147313055
Pubmed ID
36617303
Journal Title
The Journal of pharmacy and pharmacology
Volume
75
Issue
3
Start Page
427
End Page
436
Rights Holder(s)
SCOPUS
Bibliographic Citation
The Journal of pharmacy and pharmacology Vol.75 No.3 (2023) , 427-436
Suggested Citation
Boonyong C., Angkhasirisap W., Kengkoom K., Jianmongkol S. Different protective capability of chlorogenic acid and quercetin against indomethacin-induced gastrointestinal ulceration. The Journal of pharmacy and pharmacology Vol.75 No.3 (2023) , 427-436. 436. doi:10.1093/jpp/rgac098 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/82361
Title
Different protective capability of chlorogenic acid and quercetin against indomethacin-induced gastrointestinal ulceration
Author(s)
Author's Affiliation
Other Contributor(s)
Abstract
OBJECTIVES: The study compared the protective effects against indomethacin-induced GI ulceration of chlorogenic acid with quercetin in rats. METHODS: Rats were orally given chlorogenic acid or quercetin (100 mg/kg; 5 days), followed by indomethacin (40 mg/kg; single dose). After 24 h, GI tissues were assessed for histopathological damages, then analysed by ELISA and western blot methods. Cell viability was measured in vitro by MTT assay. KEY FINDINGS: Unlike quercetin, chlorogenic acid could not prevent gastric ulcers in indomethacin-treated rats. The levels of gastric prostaglandin E2 (PGE2) and Bax/Bcl-2 ratio in the chlorogenic acid-treated group were not different from those receiving indomethacin alone. Nevertheless, both compounds alleviated jejunum ulcers through suppression of PERK/eIF-2/ATF-4/CHOP-related endoplasmic reticulum (ER) stress and decrease Bax/Bcl-2 ratio. Moreover, at 100 µM, they abolished the cytotoxicity of tunicamycin (an ER stress inducer) in gastric (AGS) and intestinal (Caco-2) cells. In silico docking studies suggested that both compounds could interact with key amino acid residues in the -catalytic domain of PERK. CONCLUSION: Chlorogenic acid and quercetin exerted comparable protective effects against indomethacin-induced intestinal ulcer through suppression of ER stress-mediated apoptosis but, unlike quercetin, chlorogenic acid offered no protection against gastric ulceration due to its -inability to increase PGE2 production.