Detection of nervous necrosis virus RGNNV genotype in pearl gentian grouper (Epinephelus lanceolatus ♂ × E. fuscoguttatus ♀) fry imported to Thailand
2
Issued Date
2022-12-15
Resource Type
ISSN
00448486
Scopus ID
2-s2.0-85135103230
Journal Title
Aquaculture
Volume
561
Rights Holder(s)
SCOPUS
Bibliographic Citation
Aquaculture Vol.561 (2022)
Suggested Citation
Jungi S.V., Sangsuriya P., Taengphu S., Phiwsaiya K., Sonthi M., Nuangsaeng B., Salin K.R., Senapin S., Dong H.T. Detection of nervous necrosis virus RGNNV genotype in pearl gentian grouper (Epinephelus lanceolatus ♂ × E. fuscoguttatus ♀) fry imported to Thailand. Aquaculture Vol.561 (2022). doi:10.1016/j.aquaculture.2022.738659 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/83061
Title
Detection of nervous necrosis virus RGNNV genotype in pearl gentian grouper (Epinephelus lanceolatus ♂ × E. fuscoguttatus ♀) fry imported to Thailand
Other Contributor(s)
Abstract
Nervous necrosis virus (NNV) is a deadly virus that affects >120 fish species worldwide, but there is little information about it in Thailand. In August 2019, a population of pearl gentian grouper fry imported to Thailand experienced mass mortality. The diseased fish exhibited darkening, floating on the water surface, ‘sleepy behaviour’, and erratic swimming. PCR analysis revealed that the diseased samples were positive for NNV but negative for Megalocytivirus ISKNV and Ranavirus. Sequencing the virus's genome and phylogenetic analysis revealed that it is a member of the red-spotted grouper nervous necrosis virus (RGNNV) genotype. In situ hybridization using an NNV-specific probe revealed localization of the virus in the vacuolation lesions of the central nervous system (brain and spinal cord) and the retina of infected fish. The virus was successfully isolated from tissue homogenate from diseased fish using the E-11 cell line. This study identified NNV (RGNNV genotype) as a virus associated with massive die-offs in imported pearl grouper fry in Thailand. Considering the infectious nature of the virus and its broad host range, appropriate biosecurity measures are needed to prevent any loss to the marine aquaculture industry.