N-trans-feruloyltyramine Protects Human Neuroblastoma SK-N-SH Cell Line Against H<inf>2</inf>O<inf>2</inf>-Induced Cytotoxicity
1
Issued Date
2022-08-01
Resource Type
ISSN
1934578X
eISSN
15559475
Scopus ID
2-s2.0-85135470719
Journal Title
Natural Product Communications
Volume
17
Issue
8
Rights Holder(s)
SCOPUS
Bibliographic Citation
Natural Product Communications Vol.17 No.8 (2022)
Suggested Citation
Soi-ampornkul R., Myint E.E.P., Thangnipon W., Tantarungsee N., Mitrpant C., Tuchinda P., Nobsathian S., Vatanashevanopakorn C. N-trans-feruloyltyramine Protects Human Neuroblastoma SK-N-SH Cell Line Against H<inf>2</inf>O<inf>2</inf>-Induced Cytotoxicity. Natural Product Communications Vol.17 No.8 (2022). doi:10.1177/1934578X221117312 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/83174
Title
N-trans-feruloyltyramine Protects Human Neuroblastoma SK-N-SH Cell Line Against H<inf>2</inf>O<inf>2</inf>-Induced Cytotoxicity
Author's Affiliation
Other Contributor(s)
Abstract
Excessive accumulation of reactive oxygen species (ROS) has been regarded as a major contributor of pathogenesis in neurodegenerative diseases. N-trans-feruloyltyramine (NTF), an alkaloid isolated from several plants, has demonstrated an ability to be a potent antioxidant. In this study, the antioxidative and anti-apoptotic properties of NTF extracted from the stems of Polyalthia suberosa were investigated in the human neuroblastoma cell line SK-N-SH. NTF at concentrations ranging from 10 µM to 500 µM were not toxic to cells and reduced intracellular ROS levels significantly. Furthermore, pre-treatment of NTF significantly decreased H2O2-induced ROS generation and attenuated H2O2-mediated cytotoxicity. An increase in the expression of Bax and activated caspase-3 and reduction of Bcl-2 mediated by H2O2 was reversed by pre-treating the cells with 100 µM NTF. Likewise, NTF suppressed the increase of caspase-3 activity induced by H2O2. In conclusion, the findings reveal that NTF improves H2O2-induced intracellular ROS generation and decreases apoptosis. These protective effects of NTF could be useful for oxidative stress-related neurodegenerative conditions.