Geraniol and thymoquinone inhibit Candida spp. biofilm formation on acrylic denturresin without affecting surface roughness or color
4
Issued Date
2022-01-01
Resource Type
ISSN
13434934
eISSN
18804926
Scopus ID
2-s2.0-85128161157
Pubmed ID
35321964
Journal Title
Journal of Oral Science
Volume
64
Issue
2
Start Page
161
End Page
166
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Oral Science Vol.64 No.2 (2022) , 161-166
Suggested Citation
Kaypetch R., Rudrakanjana P., Churnjitapirom P., Tua-Ngam P., Tonput P., Tantivitayakul P. Geraniol and thymoquinone inhibit Candida spp. biofilm formation on acrylic denturresin without affecting surface roughness or color. Journal of Oral Science Vol.64 No.2 (2022) , 161-166. 166. doi:10.2334/josnusd.21-0435 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/84477
Title
Geraniol and thymoquinone inhibit Candida spp. biofilm formation on acrylic denturresin without affecting surface roughness or color
Author's Affiliation
Other Contributor(s)
Abstract
Purpose: This study was designed to investigate the in vitro effects of geraniol (GE) and thymoquinone (TQ) on Candida biofilms on denture acrylic and any accompanying changes in acrylic surface roughness or color. Methods: The susceptibility of Candida species to GE and TQ was determined using the broth microdilution method and time-kill assay. A minimum biofilm eradication concentration (MBEC) assay was performed using 7-day Candida biofilms grown on denture acrylic. Results: The minimum inhibitory concentration (MIC) of GE and TQ for Candida spp. was 256 and 32 µg/mL, respectively. The Candida strain complete kill rates for GE and TQ at 5-fold MIC were determined after 1 h of incubation. At 5-fold MIC, GE and TQ inhibited the preformed biofilm activity (MBEC80) of all Candida strains on denture acrylic by more than 80% after treatment for 3 h. At sub-MIC levels, GE and TQ prevented the development of C. albicans and C. tropicalis hyphae. SEM images demonstrated that GE and TQ damaged the fungal cell membrane and induced cell lysis. On the other hand, GE and TQ at 10-fold MIC did not alter the surface roughness or color of the denture acrylic. Conclusion: GE and TQ are interesting natural substances that could be developed as promising disinfectants for removable dentures.