Performance of the onstructural 1 Antigen Rapid Test for detecting all four DENV serotypes in clinical specimens from Bangkok, Thailand
1
Issued Date
2022-12-01
Resource Type
eISSN
1743422X
Scopus ID
2-s2.0-85140653830
Pubmed ID
36303183
Journal Title
Virology Journal
Volume
19
Issue
1
Rights Holder(s)
SCOPUS
Bibliographic Citation
Virology Journal Vol.19 No.1 (2022)
Suggested Citation
Poltep K., Phadungsombat J., Kosoltanapiwat N., Hanboonkunupakarn B., Wiriyarat W., Suwanpakdee S., Prompiram P., Nakayama E.E., Suzuki K., Iwamoto H., Shioda T., Leaungwutiwong P. Performance of the onstructural 1 Antigen Rapid Test for detecting all four DENV serotypes in clinical specimens from Bangkok, Thailand. Virology Journal Vol.19 No.1 (2022). doi:10.1186/s12985-022-01904-0 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/84845
Title
Performance of the onstructural 1 Antigen Rapid Test for detecting all four DENV serotypes in clinical specimens from Bangkok, Thailand
Other Contributor(s)
Abstract
Background:: Dengue is an arboviral disease that has a large effect on public health in subtropical and tropical countries. Rapid and accurate detection of dengue infection is necessary for diagnosis and disease management. We previously developed highly sensitive immunochromatographic devices, the TKK 1st and TKK 2nd kits, based on dengue virus (DENV) nonstructural protein 1 detection. However, these TKK kits were evaluated mainly using DENV type 2 clinical specimens collected in Bangladesh, and further validation using clinical specimens of other serotypes was needed. Methods:: In the present study, one of the TKK kits, TKK 2nd, was evaluated using 10 DENV-1, 10 DENV-2, 4 DENV-3, 16 DENV-4, and 10 zika virus-infected clinical specimens collected in Bangkok, Thailand. Results:: The TKK 2nd kit successfully detected all four DENV serotypes in patient serum specimens and did not show any cross-reactivities against zika virus serum specimens. The IgM and/or IgG anti-DENV antibodies were detected in seven serum specimens, but did not seem to affect the results of antigen detection in the TKK 2nd kit. Conclusion:: The results showed that the TKK 2nd kit successfully detected all four DENV serotypes in clinical specimens and confirmed the potential of the kit for dengue diagnosis in endemic countries.