Antigenic components, identification, and characterization of whole worm extract of Platynosomum illiciens
Issued Date
2022-10-01
Resource Type
ISSN
03044017
eISSN
18732550
Scopus ID
2-s2.0-85135964480
Pubmed ID
35981467
Journal Title
Veterinary Parasitology
Volume
310
Rights Holder(s)
SCOPUS
Bibliographic Citation
Veterinary Parasitology Vol.310 (2022)
Suggested Citation
Aoke S.M., Watthanadirek A., Poolsawat N., Srionrod N., Nooroong P., Minsakorn S., Lacharoje S., Sukhumavasi W., Anuracpreeda P. Antigenic components, identification, and characterization of whole worm extract of Platynosomum illiciens. Veterinary Parasitology Vol.310 (2022). doi:10.1016/j.vetpar.2022.109774 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/84899
Title
Antigenic components, identification, and characterization of whole worm extract of Platynosomum illiciens
Author's Affiliation
Other Contributor(s)
Abstract
The antigenic components of adult Platynosomum illiciens were characterized by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting using sera from cats naturally infected with P. illiciens, Dipylidium caninum, Toxocara cati and uninfected cat sera. The whole worm extract (WWE) of P. illiciens was fractionated by Sephadex G-200 gel filtration chromatography. The results showed that WWE fraction and F2 were highly antigenic as well as F1 and F3, which were moderately antigenic. For SDS-PAGE and immunoblotting, the antigenic molecules of WWE and all three fractions were mostly at molecular weights (MW) ranging from 11 to 150 kDa. Four antigenic proteins of 11, 18, 27 and 75 kDa detected in WWE and F1-F3 were found to give a reaction with sera from P. illiciens infected cats, and these proteins were also identified using liquid chromatography-mass spectrometry (LC-MS/MS). For immunolocalization observation, it was revealed that the P. illiciens antigen was present in high concentration in the cytoplasm of vitelline cells in the vitelline glands, the shell of the eggs and the eggs within the uterus, but not in other organs, i.e., tegument, muscle, parenchymal cells, testes and oral and ventral suckers of adult fluke. This finding indicates that these proteins may be potential antigen candidates for the immunodiagnosis of feline platynosomosis caused by P. illiciens.