Publication: Evaluation of a latex agglutination assay for the identification of Burkholderia pseudomallei and Burkholderia mallei.
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Accepted Date
2014-03-02
Issued Date
2014-04-07
Copyright Date
2014
Resource Type
Language
eng
ISSN
0002-9637 (printed)
1476-1645 (electronic)
1476-1645 (electronic)
Rights
Mahidol University
Rights Holder(s)
PubMed Cental
Bibliographic Citation
Duval BD, Elrod MG, Gee JE, Chantratita N, Tandhavanant S, Limmathurotsakul D. et al. Evaluation of a latex agglutination assay for the identificationof Burkholderia pseudomallei and Burkholderia mallei. Am J Trop Med Hyg. 2014 Jun;90(6):1043-6.
Suggested Citation
Duval, Brea D., Elrod, Mindy G., Gee, Jay E., Narisara Chantratita, นริศรา จันทราทิตย์, Sarunporn Tandhavanant, ศรัณย์พร ตันฑวนันท์, Direk Limmathurotsakul, ดิเรก ลิ้มมธุรสกุล, Hoffmaster, Alex R. Evaluation of a latex agglutination assay for the identification of Burkholderia pseudomallei and Burkholderia mallei.. Duval BD, Elrod MG, Gee JE, Chantratita N, Tandhavanant S, Limmathurotsakul D. et al. Evaluation of a latex agglutination assay for the identificationof Burkholderia pseudomallei and Burkholderia mallei. Am J Trop Med Hyg. 2014 Jun;90(6):1043-6.. doi:10.4269/ajtmh.14-0025. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/857
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Title
Evaluation of a latex agglutination assay for the identification of Burkholderia pseudomallei and Burkholderia mallei.
Corresponding Author(s)
Abstract
Cases of melioidosis and glanders are rare in the United States, but the
etiologic agents of each disease (Burkholderia pseudomallei and Burkholderia
mallei, respectively) are classified as Tier 1 select agents because of concerns
about their potential use as bioterrorism agents. A rapid, highly sensitive, and
portable assay for clinical laboratories and field use is required. Our
laboratory has further evaluated a latex agglutination assay for its ability to
identify B. pseudomallei and B. mallei isolates. This assay uses a monoclonal
antibody that specifically recognizes the capsular polysaccharide produced by B.
pseudomallei and B. mallei, but is absent in closely related Burkholderia
species. A total of 110 B. pseudomallei and B. mallei were tested, and 36 closely
related Burkholderia species. The latex agglutination assay was positive for 109
of 110 (99.1% sensitivity) B. pseudomallei and B. mallei isolates tested.