Detection of Human Papillomavirus and p16<sup>INK4a</sup> Expression in Thai Patients with Oral Squamous Cell Carcinoma
1
Issued Date
2022-06-01
Resource Type
ISSN
1936055X
eISSN
19360568
Scopus ID
2-s2.0-85116049702
Pubmed ID
34590266
Journal Title
Head and Neck Pathology
Volume
16
Issue
2
Start Page
444
End Page
452
Rights Holder(s)
SCOPUS
Bibliographic Citation
Head and Neck Pathology Vol.16 No.2 (2022) , 444-452
Suggested Citation
Rungraungrayabkul D., Panpradit N., Lapthanasupkul P., Kitkumthorn N., Klanrit P., Subarnbhesaj A., Sresumatchai V., Klongnoi B., Khovidhunkit S.o.P. Detection of Human Papillomavirus and p16<sup>INK4a</sup> Expression in Thai Patients with Oral Squamous Cell Carcinoma. Head and Neck Pathology Vol.16 No.2 (2022) , 444-452. 452. doi:10.1007/s12105-021-01381-x Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/85858
Title
Detection of Human Papillomavirus and p16<sup>INK4a</sup> Expression in Thai Patients with Oral Squamous Cell Carcinoma
Author's Affiliation
Other Contributor(s)
Abstract
This study investigated the prevalence of human papillomavirus (HPV) infection in oral squamous cell carcinoma (OSCC) cases, as well as the association between HPV presence and p16INK4a expression, in Thai patients with OSCC. Eighty-one formalin-fixed paraffin-embedded specimens of OSCC were obtained. DNA extraction was performed; this was followed by nested polymerase chain reaction analysis to determine HPV DNA status, using consensus primers for the L1 region of HPV. HPV subtypes were determined by DNA sequencing. HPV-positive specimens and HPV-negative specimens from age- and sex-matched patients were subjected to immunohistochemical analysis to determine p16INK4a expression status. Of the 81 OSCC specimens, eight (9.9%) exhibited HPV DNA; DNA sequencing confirmed that the viral subtype was HPV-18 in all eight specimens. These eight HPV-positive specimens, as well as eight HPV-negative specimens from age- and sex-matched patients, were subjected to immunohistochemical analysis to determine p16INK4a expression status. Three of eight (37.8%) HPV-positive specimens and three of eight (37.8%) HPV-negative specimens showed positive p16INK4a expression findings. However, we did not find a significant association between HPV status and p16INK4a expression status in our OSCC samples. In conclusion, the prevalence of high-risk HPV was low in this group of OSCC patients; no association between HPV status and p16INK4a expression status was identified.