Chemometrics-assisted spectroscopic methods for rapid analysis of combined anti-malarial tablets
Issued Date
2023-06-15
Resource Type
eISSN
22246614
Scopus ID
2-s2.0-85162624451
Pubmed ID
37335160
Journal Title
Journal of food and drug analysis
Volume
31
Issue
2
Start Page
338
End Page
357
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of food and drug analysis Vol.31 No.2 (2023) , 338-357
Suggested Citation
Pruksapha P., Khongkaew P., Suwanvecho C., Nuchtavorn N., Phechkrajang C., Suntornsuk L. Chemometrics-assisted spectroscopic methods for rapid analysis of combined anti-malarial tablets. Journal of food and drug analysis Vol.31 No.2 (2023) , 338-357. 357. doi:10.38212/2224-6614.3449 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/87805
Title
Chemometrics-assisted spectroscopic methods for rapid analysis of combined anti-malarial tablets
Author's Affiliation
Other Contributor(s)
Abstract
Combination of piperaquine (PQ) (320mg) and dihydroartemisinin (DHA) (40 mg) is an anti-malarial formulation, which is recommended by World Health Organization (WHO). Simultaneous analysis of PQ and DHA can be problematic due to the lack of chromophores or fluorophores in DHA molecule. Whereas PQ possesses strong UV absorption and it presents in 8 times of DHA contents in the formulation. In this study, two spectroscopic methods, Fourier transform infrared (FTIR) and Raman spectroscopy, were developed for the determination of both drugs in combined tablets. The FTIR and Raman spectra were recorded in the attenuate total reflectance (ATR) and scattering modes, respectively. The original and pretreated spectra from FTIR and handheld-Raman were subjected to Unscrambler® program to construct partial least squares regression (PLSR) model comparing with references values obtained from high performance liquid chromatography (HPLC)-UV method. The optimal PLSR models of PQ and DHA from FTIR spectroscopy were obtained from orthogonal signal correction (OSC) pretreatment at the wavenumbers 400-1,800 cm-1 and 1,400-4,000 cm-1, respectively. For Raman spectroscopy of PQ and DHA, the optimal PLSR models were obtained from standard normal variate (SNV) pretreatment at the wavenumbers 1,200-2,300 cm-1 and OSC pretreatment at the wavenumber 400-2,300 cm-1, respectively. Determination of PQ and DHA in tablets from the optimum model was compared with HPLC-UV method. Results were not significantly different at 95% confidence limit (p-value >0.05). The chemometrics-assisted spectroscopic methods were fast (1-3 min), economical and less labor intensive. Moreover, the handheld Raman spectrometer is portable and can be utilized for onsite analysis to facilitate the detection of counterfeit or substandard drugs at ports of entry.