Biological Activities and Crystal Structure Determination of trans, trans-Cyclohexane-1,2,4,5-tetrol Monohydrate from Pseuduvaria phuyensis (R.M.K. Saunders)
5
Issued Date
2023-07-31
Resource Type
ISSN
26160684
eISSN
26160692
Scopus ID
2-s2.0-85166566037
Journal Title
Tropical Journal of Natural Product Research
Volume
7
Issue
7
Start Page
3314
End Page
3319
Rights Holder(s)
SCOPUS
Bibliographic Citation
Tropical Journal of Natural Product Research Vol.7 No.7 (2023) , 3314-3319
Suggested Citation
Sukyod R., Udomputtimekakul P., Pompimon W., Narakaew S., Jiajaroen S., Chainok K., Laohakul C., Khonghuayrob S., Chaisena A., Wattananon S., Nuntasaen N., Suksen K., Chairoungdua A., Limthongkul J., Naparswad C., Charoenphakinrattana N., Pikulthong S. Biological Activities and Crystal Structure Determination of trans, trans-Cyclohexane-1,2,4,5-tetrol Monohydrate from Pseuduvaria phuyensis (R.M.K. Saunders). Tropical Journal of Natural Product Research Vol.7 No.7 (2023) , 3314-3319. 3319. doi:10.26538/tjnpr/v7i7.7 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/88306
Title
Biological Activities and Crystal Structure Determination of trans, trans-Cyclohexane-1,2,4,5-tetrol Monohydrate from Pseuduvaria phuyensis (R.M.K. Saunders)
Author's Affiliation
Other Contributor(s)
Abstract
Pseuduvaria phuyensis (Annonaceae), was collected from Kanchanaburi Province, western Thailand. The study aimed to evaluate the antibacterial, anti-HIV-1RT, anti-syncytium (MC99+1A2), and cytotoxic potentials of the crude hexane, ethyl-acetate, and methanol extracts of P. phuyensis leaf-twigs and stems, all in comparison with standard drugs. Also, the isolation and characterization of a compound from one of the active extracts as well as its antibacterial and cytotoxicity activities were carried out. The MIC and MBC values of selected bacterial strains ranged from 3.125 – 200 and 6.25 – 200 mg/mL, respectively for all the extracts, while the isolated compound was not active. The anti-HIV-1 RT and the anti-syncytium assay of the hexane and ethyl acetate extracts revealed they were very active, with % inhibitions of 90.50 and 89.36, respectively. The best inhibitory concentration at 50% (IC50) value was 44.33 µM as exhibited by the hexane extract of the stems of the reverse transcriptase assay, while, the best effective concentration at 50% (EC50) value was 10.4 µM (TI>4.83) as displayed by the ethyl acetate extract of the stems. The cytotoxicity study showed that the hexane extract of the stems displayed high cytotoxicity with ED50 value at 13.07 µg/mL against the FaDu cancer cell line, whil that of the isolated compound was not active. The structure of the isolated compound was characterized and elucidated as trans, trans-cyclohexane-1, 2, 4, 5-tetrol monohydrate using spectroscopic technique and X-ray crystallography analysis.