Andrographolide Induces ROS-Mediated Cytotoxicity, Lipid Peroxidation, and Compromised Cell Integrity in Saccharomyces cerevisiae
1
Issued Date
2023-09-01
Resource Type
eISSN
20763921
Scopus ID
2-s2.0-85172285982
Journal Title
Antioxidants
Volume
12
Issue
9
Rights Holder(s)
SCOPUS
Bibliographic Citation
Antioxidants Vol.12 No.9 (2023)
Suggested Citation
Phetruen T., van Dam B., Chanarat S. Andrographolide Induces ROS-Mediated Cytotoxicity, Lipid Peroxidation, and Compromised Cell Integrity in Saccharomyces cerevisiae. Antioxidants Vol.12 No.9 (2023). doi:10.3390/antiox12091765 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/90285
Title
Andrographolide Induces ROS-Mediated Cytotoxicity, Lipid Peroxidation, and Compromised Cell Integrity in Saccharomyces cerevisiae
Author(s)
Author's Affiliation
Other Contributor(s)
Abstract
Andrographolide, a bioactive compound found in Andrographis paniculata, has gained significant attention for its potential therapeutic properties. Despite its promising benefits, the understanding of its side effects and underlying mechanisms remains limited. Here, we investigated the impact of andrographolide in Saccharomyces cerevisiae and observed that andrographolide induced cytotoxicity, particularly when oxidative phosphorylation was active. Furthermore, andrographolide affected various cellular processes, including vacuole fragmentation, endoplasmic reticulum stress, lipid droplet accumulation, reactive oxygen species levels, and compromised cell integrity. Moreover, we unexpectedly observed that andrographolide induced the precipitation of biomolecules secreted from yeast cells, adding an additional source of stress. Overall, this study provides insights into the cellular effects and potential mechanisms of andrographolide in yeast, shedding light on its side effects and underlying cytotoxicity pathways.