Publication: Cloning and characterization of a novel Plasmodium falciparum sporozoite surface antigen, STARP
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Issued Date
1994-01-01
Resource Type
ISSN
01666851
Other identifier(s)
2-s2.0-0028302559
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Mahidol University
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SCOPUS
Bibliographic Citation
Molecular and Biochemical Parasitology. Vol.64, No.2 (1994), 219-232
Suggested Citation
David A. Fidock, Emmanuel Bottius, Karima Brahimi, I. M.D. Inge, Moelans, Masamichi Aikawa, Ruud N.H. Konings, Ulrich Certa, Petur Olafsson, Toshiyuki Kaidoh, Achara Asavanich, Claudine Guerin-Marchand, Pierre Druilhe Cloning and characterization of a novel Plasmodium falciparum sporozoite surface antigen, STARP. Molecular and Biochemical Parasitology. Vol.64, No.2 (1994), 219-232. doi:10.1016/0166-6851(94)00012-3 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/9543
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Title
Cloning and characterization of a novel Plasmodium falciparum sporozoite surface antigen, STARP
Abstract
A novel Plasmodium falciparum sporozoite antigen, STARP (Sporozoite Threonine and Asparagine-Rich Protein), detected consistently on the surface of sporozoites obtained from laboratory strains and field isolates, has been identified and cloned, following a systematic approach aimed at isolating novel non-CS sporozoite surface antigens. The 2.0-kb STARP gene has a 5′ miniexon/large central exon structure and contains a complex repetitive region encoding multiple dispersed motifs and tandem 45- and 10-amino acid repeats. In sporozoites, transcription of the STARP gene has been conclusively demonstrated by reverse PCR and Northern blot hybridisation and the 78-kDa protein has been localized by immunofluorescence and immunoelectron microscopy to the sporozoite surface. STARP is also expressed in liver stages, as revealed by immunofluorescence assays using antisera raised either to the central repetitive region or the C-terminal non-repetitive region. Expression is also detected in early ring stages, though not in mature erythrocytic or sexual stages. Identification and elucidation of this novel antigen is a step forward in current efforts aimed at developing an effective preerythrocytic-stage malaria vaccine. © 1994.