Publication: Transfer of plasmids pBC 16 and pC 194 into Bacillus thuringiensis subsp. israelensis
Issued Date
1986-01-01
Resource Type
ISSN
10960805
00222011
00222011
Other identifier(s)
2-s2.0-46149132239
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Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Invertebrate Pathology. Vol.48, No.3 (1986), 325-334
Suggested Citation
Suvit Loprasert, Somsak Pantuwatana, Amaret Bhumiratana Transfer of plasmids pBC 16 and pC 194 into Bacillus thuringiensis subsp. israelensis. Journal of Invertebrate Pathology. Vol.48, No.3 (1986), 325-334. doi:10.1016/0022-2011(86)90061-3 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/9649
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Title
Transfer of plasmids pBC 16 and pC 194 into Bacillus thuringiensis subsp. israelensis
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Abstract
A lysozyme sensitive strain of B. thuringiensis (strain O 016) was isolated and shown to be effectively transformed with plasmids pC 194 and pHV 33 using the protoplast transformation technique. The plasmid pC 194 from one successful transformant, strain O 016-194, was subsequently transferred to B. thuringiensis subsp. israelensis by a "conjugation-like" process. The plasmid pBC 16 from B. cereus could also be transferred to B. thuringiensis subsp. israelensis with high frequency using the conjugation-like process. Further, both plasmids, pC 194 and pBC 16, were transferred between strains of B. thuringiensis subsp. israelensis to yield transcipient strains that harbored and expressed properties of both plasmids. This work constitutes effective gene transfer system in B. thuringiensis subsp. israelensis. © 1986.