Cyanogenesis of cassava plantlets in tissue culture
1
Issued Date
2024
Copyright Date
1991
Resource Type
Language
eng
File Type
application/pdf
No. of Pages/File Size
x, 90 leaves : ill.
Access Rights
open access
Rights
ผลงานนี้เป็นลิขสิทธิ์ของมหาวิทยาลัยมหิดล ขอสงวนไว้สำหรับเพื่อการศึกษาเท่านั้น ต้องอ้างอิงแหล่งที่มา ห้ามดัดแปลงเนื้อหา และห้ามนำไปใช้เพื่อการค้า
Rights Holder(s)
Mahidol University
Bibliographic Citation
Thesis (M.Sc. (Biochemistry))--Mahidol University, 1991
Suggested Citation
Panomporn Panutat Cyanogenesis of cassava plantlets in tissue culture. Thesis (M.Sc. (Biochemistry))--Mahidol University, 1991. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/99093
Title
Cyanogenesis of cassava plantlets in tissue culture
Alternative Title(s)
ไซยาโนเจเนซิสในมันสำปะหลัง
Author(s)
Advisor(s)
Abstract
Cassava plantlets grown in Murashige and Skoog medium were shown to contain linamarin. The linamarin was found mainly in the leaf and the petiole with small amount in the stem and the root. The linamarin content increased with age reaching a maximum after 5 months. The content was not affected by the level of the minerals in the medium or addition of valine, the known precursor of linamarin, until a very high level of valine was used. Linamarase was found in the plantlets. Its specific activity was highest in the leaf of plantlets at 3 months of age peroxidase was also found in the plantlets. Its specific activity was highest in the root of 5 mohths old plantlets. Radioactive incorporation from U - [(14)C] - L - valine into linamarin by petiole slices was higher than that by leaf slices. The incorporation by the petiole slices increased upon the addition of a linamarase inhibitor, isopropyl - β - D - thioglucopyranoside or δ- gluconolactone, but not by glucos fructose or starch. The finding suggested the potential use of cassava plant tissue culture in studying the control of cyanogenic glucoside metabolism.
Description
Biochemistry (Mahidol University 1991)
Degree Name
Master of Science
Degree Level
Master's degree
Degree Department
Faculty of Science
Degree Discipline
Biochemistry
Degree Grantor(s)
Mahidol University