Nanozyme-Shelled Microcapsules for Targeting Biofilm Infections in Confined Spaces
Issued Date
2024-01-01
Resource Type
ISSN
21922640
eISSN
21922659
Scopus ID
2-s2.0-85206188782
Journal Title
Advanced Healthcare Materials
Rights Holder(s)
SCOPUS
Bibliographic Citation
Advanced Healthcare Materials (2024)
Suggested Citation
Tran H.H., Jaruchotiratanasakul N., Xiang Z., Pandey N.K., Oh M.J., Liu Y., Ren Z., Babeer A., Zdilla M.J., Cormode D.P., Karabucak B., Lee D., Steager E.B., Koo H. Nanozyme-Shelled Microcapsules for Targeting Biofilm Infections in Confined Spaces. Advanced Healthcare Materials (2024). doi:10.1002/adhm.202402306 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/101673
Title
Nanozyme-Shelled Microcapsules for Targeting Biofilm Infections in Confined Spaces
Corresponding Author(s)
Other Contributor(s)
Abstract
Bacterial infections in irregular and branched confinements pose significant therapeutic challenges. Despite their high antimicrobial efficacy, enzyme-mimicking nanoparticles (nanozymes) face difficulties in achieving localized catalysis at distant infection sites within confined spaces. Incorporating nanozymes into microrobots enables the delivery of catalytic agents to hard-to-reach areas, but poor nanoparticle dispersibility and distribution during fabrication hinder their catalytic performance. To address these challenges, a nanozyme-shelled microrobotic platform is introduced using magnetic microcapsules with collective and adaptive mobility for automated navigation and localized catalysis within complex confinements. Using double emulsions produced from microfluidics as templates, iron oxide and silica nanoparticles are assembled into 100-µm microcapsules, which self-organize into multi-unit, millimeter-size assemblies under rotating magnetic fields. These microcapsules exhibit high peroxidase-like activity, efficiently catalyzing hydrogen peroxide to generate reactive oxygen species (ROS). Notably, microcapsule assemblies display remarkable collective navigation within arched and branched confinements, reaching the targeted apical regions of the tooth canal with high accuracy. Furthermore, these nanozyme-shelled microrobots perform rapid catalysis in situ and effectively kill biofilms on contact via ROS generation, enabling localized antibiofilm action. This study demonstrates a facile method of integrating nanozymes onto a versatile microrobotic platform to address current needs for targeted therapeutic catalysis in complex and confined microenvironments.