Optimizing the production of recombinant human papilloma virus type 52 major capsid protein L1 in Hansenula polymorpha
Issued Date
2024-12-01
Resource Type
eISSN
20452322
Scopus ID
2-s2.0-85209593327
Journal Title
Scientific Reports
Volume
14
Issue
1
Rights Holder(s)
SCOPUS
Bibliographic Citation
Scientific Reports Vol.14 No.1 (2024)
Suggested Citation
Phimsen W., Kopitak N., Boontawon T., Theeranan T., Boonchird C., Pongtharangkul T. Optimizing the production of recombinant human papilloma virus type 52 major capsid protein L1 in Hansenula polymorpha. Scientific Reports Vol.14 No.1 (2024). doi:10.1038/s41598-024-79764-7 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/102168
Title
Optimizing the production of recombinant human papilloma virus type 52 major capsid protein L1 in Hansenula polymorpha
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Abstract
Cervical cancer is the fourth most prevalent cancer among women globally, with Thai women ranking it as the third most common. At present, a prophylactic vaccine, containing virus-like particles (VLPs) of HPV L1 capsid protein, is widely recognized as one of the major prevention strategies for cervical cancer. Unfortunately, due to a low cross-protection among subtypes, protection against each HPV subtype requires vaccination with VLPs of that specific subtype. This study aimed to optimize the cultivation medium and conditions to maximize the volumetric yield of HPV52 L1 protein produced by the recombinant H. polymorpha HPV52. The results revealed that supplementation of a complex organic nitrogen source HySoy (at 10 g/L) into SYN6 medium resulted in a significantly higher specific HPV52 L1 yield and the maximum specific and volumetric HPV52 L1 protein yield were obtained at 30℃. In this study, the volumetric yield of HPV52 L1 could be increased from 50.9 mg/L in flask-scale cultivation to 2728 mg/L in High Cell Density Cultivation or HCDC (53-folds) with a significant improvement in terms of productivity, from 0.85 mg/L.h in flask-scale cultivation to 22.7 mg/L.h in HCDC (27-folds).